Yoo, S.K., Freisinger, C.M., Lebert, D.C., and Huttenlocher, A. (2012) Early redox, Src family kinase, and calcium signaling integrate wound responses and tissue regeneration in zebrafish. The Journal of cell biology. 199(2):225-234.
Tissue injury can lead to scar formation or tissue regeneration. How regenerative animals sense initial tissue injury and
transform wound signals into regenerative growth is an unresolved question. Previously, we found that the Src family kinase
(SFK) Lyn functions as a redox sensor in leukocytes that detects H2O2 at wounds in zebrafish larvae. In this paper, using zebrafish larval tail fins as a model, we find that wounding rapidly
activated SFK and calcium signaling in epithelia. The immediate SFK and calcium signaling in epithelia was important for late
epimorphic regeneration of amputated fins. Wound-induced activation of SFKs in epithelia was dependent on injury-generated
H2O2. A SFK member, Fynb, was responsible for fin regeneration. This work provides a new link between early wound responses and
late regeneration and suggests that redox, SFK, and calcium signaling are immediate “wound signals” that integrate early wound
responses and late epimorphic regeneration.