Action of Vitamin D and the Receptor, VDRa, in Calcium Handling in Zebrafish (Danio rerio)
- Authors
- Lin, C.H., Su, C.H., Tseng, D.Y., Ding, F.C., and Hwang, P.P.
- ID
- ZDB-PUB-121012-17
- Date
- 2012
- Source
- PLoS One 7(9): e45650 (Journal)
- Registered Authors
- Hwang, Pung Pung, Tseng, Deng-Yu
- Keywords
- Zebrafish, Embryos, Vitamin D, Gene expression, Vitamins, Vertebrates, Fresh water, Mammals
- MeSH Terms
-
- DNA Primers
- Amino Acid Sequence
- Real-Time Polymerase Chain Reaction
- Base Sequence
- Calcium/metabolism*
- Molecular Sequence Data
- Immunohistochemistry
- Zebrafish
- Gene Knockdown Techniques
- Animals
- Reverse Transcriptase Polymerase Chain Reaction
- RNA, Messenger/genetics
- TRPV Cation Channels/genetics
- TRPV Cation Channels/physiology
- Sodium-Potassium-Exchanging ATPase/metabolism
- Sequence Homology, Amino Acid
- Vitamin D/genetics
- Vitamin D/physiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology
- Receptors, Calcitriol/chemistry
- Receptors, Calcitriol/genetics
- Receptors, Calcitriol/physiology*
- PubMed
- 23029160 Full text @ PLoS One
The purpose of the present study was to use zebrafish as a model to investigate how vitamin D and its receptors interact to control Ca2+ uptake function. Low-Ca2+ fresh water stimulated Ca2+ influx and expressions of epithelial calcium channel (ecac), vitamin D-25-hydroxylase (cyp2r1), vitamin D receptor a (vdra), and vdrb in zebrafish. Exogenous vitamin D increased Ca2+ influx and expressions of ecac and 25-hydroxyvitamin D3-24-hydroxylase (cyp24a1), but downregulated 1α-OHase (cyp27b1) with no effects on other Ca2+ transporters. Morpholino oligonucleotide knockdown of VDRa, but not VDRb, was found as a consequence of calcium uptake inhibition by knockdown of ecac, and ossification of vertebrae is impaired. Taken together, vitamin D-VDRa signaling may stimulate Ca2+ uptake by upregulating ECaC in zebrafish, thereby clarifying the Ca2+-handling function of only a VDR in teleosts. Zebrafish may be useful as a model to explore the function of vitamin D-VDR signaling in Ca2+ homeostasis and the related physiological processes in vertebrates.