PUBLICATION

Reverse genetic approaches in zebrafish

Authors
Huang, P., Zhu, Z., Lin, S., and Zhang, B.
ID
ZDB-PUB-121010-21
Date
2012
Source
Journal of genetics and genomics = Yi chuan xue bao   39(9): 421-433 (Review)
Registered Authors
Huang, Peng, Lin, Shuo, Zhang, Bo, Zhu, Zuoyan
Keywords
zebrafish, reverse genetics, morpholino, TILLING, retrovirus, ZFN, TALEN, gene targeting
MeSH Terms
  • Animals
  • Ethylnitrosourea/toxicity
  • Gene Expression Regulation/genetics*
  • Gene Targeting
  • Genome
  • INDEL Mutation/drug effects*
  • Mutagenesis, Site-Directed
  • Reverse Genetics*
  • Zebrafish/genetics*
  • Zinc Fingers/genetics
PubMed
23021542 Full text @ J. Genet. Genomics
Abstract

Zebrafish (Danio rerio) is a well-established vertebrate animal model. A comprehensive collection of reverse genetics tools has been developed for studying gene function in this useful organism. Morpholino is the most widely used reagent to knock down target gene expression post-transcriptionally. For a long time, targeted genome modification has been heavily relied on large-scale traditional forward genetic screens, such as ENU (N-ethyl-N-nitrosourea) mutagenesis derived TILLING (Targeting Induced Local Lesions IN Genomes) strategy and pseudo-typed retrovirus mediated insertional mutagenesis. Recently, engineered endonucleases, including ZFNs (zinc finger nucleases) and TALENs (transcription activator-like effector nucleases), provide new and efficient strategies to directly generate site-specific indel mutations by inducing double strand breaks in target genes. Here we summarize the major reverse genetic approaches for loss-of-function studies used and emerging in zebrafish, including strategies based on genome-wide mutagenesis and methods for site-specific gene targeting. Future directions and expectations will also be discussed.

Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes