Analysis of protein expression in developmental toxicity induced by MeHg in zebrafish
- Authors
- Cuello, S., Ximénez-Embún, P., Ruppen, I., Schonthaler, H.B., Ashman, K., Madrid, Y., Luque-Garcia, J.L., and Cámara, C.
- ID
- ZDB-PUB-121005-22
- Date
- 2012
- Source
- The Analyst 137(22): 5302-5311 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Calcium/metabolism
- Chromatography, High Pressure Liquid*
- Chromatography, Ion Exchange
- Chromatography, Reverse-Phase
- Embryonic Development/drug effects*
- Energy Metabolism
- Isoelectric Focusing
- Larva/drug effects
- Larva/growth & development
- Methylmercury Compounds/chemistry
- Methylmercury Compounds/toxicity*
- Peptides/analysis
- Proteome/analysis*
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*
- Zebrafish/growth & development
- PubMed
- 23014190 Full text @ Analyst
Mercury toxicity and its implications in development are a major concern, due to the major threat to ecosystems and human health that this compound represents. Although some of the effects of methylmercury (MeHg) exposure have been extensively studied, the molecular mechanisms of interaction between this compound and developing organisms are still not completely understood. To provide further insights into these mechanisms, we carried out a quantitative proteomic study (iTRAQ) using zebrafish larvae exposed to 5 μg L1 and 25 μg L1 MeHg as a model. In this study, a multidimensional approach combining isoelectric focusing (IEF) and strong cation exchange (SCX) followed by reversed phase liquid chromatography prior to MALDI TOF/TOF analysis was employed, which resulted in a substantial increase in proteome coverage. Among the proteins identified, 71 were found de-regulated by more than 1.5-fold, and implicated in embryonic development, protein synthesis, calcium homeostasis and energy production. Furthermore, morphological and histological analysis of exposed larvae was carried out, reflecting changes such as smaller swim bladder, remaining yolk, bent body axis and accumulation of blood in the heart, among others.