PUBLICATION
Manipulation of gene expression in zebrafish using caged circular morpholino oligomers
- Authors
- Wang, Y., Wu, L., Wang, P., Lv, C., Yang, Z., and Tang, X.
- ID
- ZDB-PUB-121005-2
- Date
- 2012
- Source
- Nucleic acids research 40(21): 11155-11162 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/metabolism
- Fetal Proteins
- Gene Expression Regulation*/radiation effects
- Morpholinos/chemical synthesis
- Morpholinos/chemistry*
- Morpholinos/radiation effects
- Oligodeoxyribonucleotides/chemistry
- Photolysis
- T-Box Domain Proteins/genetics
- T-Box Domain Proteins/metabolism
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- beta Catenin/genetics
- beta Catenin/metabolism
- PubMed
- 23002141 Full text @ Nucleic Acids Res.
Citation
Wang, Y., Wu, L., Wang, P., Lv, C., Yang, Z., and Tang, X. (2012) Manipulation of gene expression in zebrafish using caged circular morpholino oligomers. Nucleic acids research. 40(21):11155-11162.
Abstract
Morpholino oligomers (MOs) have been widely used to knock down specific genes in zebrafish, but their constitutive activities limit their experimental applications for studying a gene with multiple functions or within a gene network. We report herein a new design and synthesis of caged circular MOs (caged cMOs) with two ends linked by a photocleavable moiety. These caged cMOs were successfully used to photomodulate β-catenin-2 and no tail expression in zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping