PUBLICATION

Arginine exposure alters ectonucleotidase activities and morphology of zebrafish larvae (Danio rerio)

Authors
Capiotti, K.M., Fazenda, L., Nazario, L.R., Menezes, F.P., Kist, L.W., Bogo, M.R., Da Silva, R.S., Wyse, A.T., and Bonan, C.D.
ID
ZDB-PUB-121004-10
Date
2013
Source
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience   31(1): 75-81 (Journal)
Registered Authors
Bonan, Carla Denise
Keywords
arginine, NTPDase, Ecto-5'-nucleotidase, development, hyperargininemia, zebrafish
MeSH Terms
  • Adenosine Diphosphate/metabolism
  • Adenosine Monophosphate/metabolism
  • Adenosine Triphosphate/metabolism
  • Age Factors
  • Analysis of Variance
  • Animals
  • Arginine/pharmacology*
  • Brain/drug effects
  • Brain/enzymology
  • Brain/ultrastructure
  • Cell Membrane/drug effects
  • Cell Membrane/enzymology
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation, Developmental/drug effects
  • Gene Expression Regulation, Enzymologic/drug effects
  • Larva/drug effects*
  • Motor Activity/drug effects
  • Nucleotidases/metabolism*
  • Zebrafish
PubMed
22995533 Full text @ Int. J. Dev. Neurosci.
Abstract

Hyperargininemia is an inborn error of metabolism (IEM) characterized by tissue accumulation of arginine (Arg). Mental retardation and other neurological features are common symptoms in hyperargininemic patients. Considering purinergic signaling has a crucial role from the early stages of development and underlying mechanisms of this disease are poorly established, we investigated the effect of Arg administration on locomotor activity, morphological alterations, and extracellular nucleotide hydrolysis in larvae and adult zebrafish. We showed that 0.1 mM Arg was unable to promote changes in locomotor activity. In addition, 7-day-post-fertilization (dpf) larvae treated with Arg demonstrated a decreased body size. Arg exposure (0.1 mM) promoted an increase in ATP, ADP, and AMP hydrolysis when compared to control group. These findings demonstrated that Arg might affect morphological parameters and ectonucleotidase activities in zebrafish larvae, suggesting that purinergic system is a target for neurotoxic effects induced by Arg.

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