Antimicrobial-immunomodulatory activities of zebrafish phosvitin-derived peptide Pt5
- Authors
- Ding, Y., Liu, X., Bu, L., Li, H., and Zhang, S.
- ID
- ZDB-PUB-120807-2
- Date
- 2012
- Source
- Peptides 37(2): 309-313 (Journal)
- Registered Authors
- Li, Hongyan
- Keywords
- zebrafish, Danio rerio, phosvitin, antimicrobial peptide, immunomodulator
- MeSH Terms
-
- Aeromonas hydrophila/drug effects*
- Animals
- Anti-Bacterial Agents/chemistry
- Anti-Bacterial Agents/immunology
- Anti-Bacterial Agents/pharmacology*
- Immunomodulation*/drug effects
- Microbial Sensitivity Tests
- Peptide Fragments/chemistry
- Peptide Fragments/immunology*
- Peptide Fragments/pharmacology*
- Phosvitin/chemistry*
- Phosvitin/immunology
- Phosvitin/pharmacology
- Structure-Activity Relationship
- Zebrafish*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/immunology*
- Zebrafish Proteins/pharmacology*
- PubMed
- 22841856 Full text @ Peptides
A phosvitin (Pv)-derived peptide, Pt5, which consists of the C-terminal 55 residues of Pv in zebrafish, has been shown to function as an antimicrobial agent capable of killing microbes in vitro. However, its in vivo role in zebrafish remains unknown. In this study, we clearly demonstrated that Pt5 protected adult zebrafish from pathogenic Aeromonas hydrophilia attack, capable of significantly enhancing the survival rate of zebrafish after the pathogenic challenge. Pt5 also caused a marked decrease in the numbers of A. hydrophilia in the blood, spleen, kidney, liver and muscle, suggesting that Pt5 was able to block multiplication/dissemination of A. hydrophilia in zebrafish. Additionally, Pt5 markedly suppressed the expression of the proinflammatory cytokine genes IL-1β, IL-6, TNF-α and IFN-γ in the spleen and head kidney of A. hydrophilia-infected zebrafish, but it considerably enhanced the expressions of the antiinflammatory cytokine genes IL-10 and IL-4 in the same tissues. Taken together, these data indicate that Pt5 plays a dual role in zebrafish as an antimicrobial and immunomodulatory agent, capable of protecting zebrafish against pathogenic A. hydrophilia through its antimicrobial activity as well as preventing zebrafish from the detrimental effects of an excessive inflammatory response via modulating immune functions.