PUBLICATION

Marine Cyclotripeptide X-13 Promotes Angiogenesis in Zebrafish and Human Endothelial Cells via PI3K/Akt/eNOS Signaling Pathways

Authors
Lu, X.L., Xu, Z.L., Yao, X.L., Su, F.J., Ye, C.H., Li, J., Lin, Y.C., Wang, G.L., Zeng, J.S., Huang, R.X., Ou, J.S., Sun, H.S., Wang, L.P., Pang, J.Y., and Pei, Z.
ID
ZDB-PUB-120724-34
Date
2012
Source
Marine drugs   10(6): 1307-1320 (Journal)
Registered Authors
Keywords
angiogenesis, marine cyclotripeptide, zebrafish, endothelial nitric oxide synthase, xyloallenoide A
MeSH Terms
  • Chromones/pharmacology
  • Aquatic Organisms/chemistry*
  • Signal Transduction/drug effects*
  • Neovascularization, Physiologic/drug effects*
  • Phosphorylation/drug effects
  • Proto-Oncogene Proteins c-akt/antagonists & inhibitors
  • Proto-Oncogene Proteins c-akt/metabolism*
  • Biological Products/chemical synthesis
  • Biological Products/chemistry
  • Biological Products/isolation & purification
  • Biological Products/pharmacology
  • Nitric Oxide Synthase Type III/antagonists & inhibitors
  • Nitric Oxide Synthase Type III/metabolism*
  • Cell Line
  • Nitric Oxide/metabolism
  • Endothelial Cells/drug effects
  • Endothelial Cells/metabolism
  • Zebrafish/metabolism
  • Humans
  • Angiogenesis Inducing Agents/chemical synthesis
  • Angiogenesis Inducing Agents/chemistry
  • Angiogenesis Inducing Agents/isolation & purification
  • Angiogenesis Inducing Agents/pharmacology*
  • Morpholines/pharmacology
  • NG-Nitroarginine Methyl Ester/pharmacology
  • Human Umbilical Vein Endothelial Cells
  • Phosphatidylinositol 3-Kinases/antagonists & inhibitors
  • Phosphatidylinositol 3-Kinases/metabolism*
  • Fungi/chemistry
  • Animals
PubMed
22822374 Full text @ Mar. Drugs
Abstract

Cyclotripeptide X-13 is a core of novel marine compound xyloallenoide A isolated from mangrove fungus Xylaria sp. (no. 2508). We found that X-13 dose-dependently induced angiogenesis in zebrafish embryos and in human endothelial cells, which was accompanied by increased phosphorylation of eNOS and Akt and NO release. Inhibition of PI3K/Akt/eNOS by LY294002 or L-NAME suppressed X-13-induced angiogenesis. The present work demonstrates that X-13 promotes angiogenesis via PI3K/Akt/eNOS pathways.

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