Carbonic anhydrase 5 regulates Acid-base homeostasis in zebrafish
- Authors
- Postel, R., and Sonnenberg, A.
- ID
- ZDB-PUB-120706-2
- Date
- 2012
- Source
- PLoS One 7(6): e39881 (Journal)
- Registered Authors
- Postel, Ruben
- Keywords
- none
- MeSH Terms
-
- Acid-Base Equilibrium/genetics
- Acid-Base Equilibrium/physiology
- Animals
- Carbonic Anhydrase V/genetics
- Carbonic Anhydrase V/metabolism*
- Embryonic Development
- Homeostasis/genetics
- Homeostasis/physiology
- Hydrogen-Ion Concentration
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 22745834 Full text @ PLoS One
The regulation of the acid-base balance in cells is essential for proper cellular homeostasis. Disturbed acid-base balance directly affects cellular physiology, which often results in various pathological conditions. In every living organism, the protein family of carbonic anhydrases regulate a broad variety of homeostatic processes. Here we describe the identification, mapping and cloning of a zebrafish carbonic anhydrase 5 (ca5) mutation, collapse of fins (cof), which causes initially a collapse of the medial fins followed by necrosis and rapid degeneration of the embryo. These phenotypical characteristics can be mimicked in wild-type embryos by acetazolamide treatment, suggesting that CA5 activity in zebrafish is essential for a proper development. In addition we show that CA5 regulates acid-base balance during embryonic development, since lowering the pH can compensate for the loss of CA5 activity. Identification of selective modulators of CA5 activity could have a major impact on the development of new therapeutics involved in the treatment of a variety of disorders.