Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging
- Zeng, Y., Xu, J., Li, D., Li, L., Wen, Z., and Qu, J.Y.
- Optics letters 37(13): 2490-2492 (Journal)
- Registered Authors
- Wen, Zilong
- MeSH Terms
- Blood Circulation
- Flow Cytometry/methods*
- Molecular Imaging/methods*
- Spectrometry, Fluorescence/methods*
- 22743431 Full text @ Opt. Lett.
Zeng, Y., Xu, J., Li, D., Li, L., Wen, Z., and Qu, J.Y. (2012) Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging. Optics letters. 37(13):2490-2492.
We demonstrate a label-free in vivo flow cytometry in zebrafish blood vessels based on two-photon excited autofluorescence imaging. The major discovery in this work is the strong autofluorescence emission from the plasma in zebrafish blood. The plasma autofluorescence provides excellent contrast for visualizing blood vessels and counting blood cells. In addition, the cellular nicotinamide adenine dinucleotide autofluorescence enables in vivo imaging and counting of white blood cells (neutrophils).
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes