ZFIN ID: ZDB-PUB-120705-28
Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging
Zeng, Y., Xu, J., Li, D., Li, L., Wen, Z., and Qu, J.Y.
Date: 2012
Source: Optics letters   37(13): 2490-2492 (Journal)
Registered Authors: Wen, Zilong
Keywords: none
MeSH Terms:
  • Animals
  • Blood Circulation
  • Erythrocytes/cytology
  • Flow Cytometry/methods*
  • Leukocytes/cytology
  • Molecular Imaging/methods*
  • Photons*
  • Spectrometry, Fluorescence/methods*
  • Zebrafish*/physiology
PubMed: 22743431 Full text @ Opt. Lett.

We demonstrate a label-free in vivo flow cytometry in zebrafish blood vessels based on two-photon excited autofluorescence imaging. The major discovery in this work is the strong autofluorescence emission from the plasma in zebrafish blood. The plasma autofluorescence provides excellent contrast for visualizing blood vessels and counting blood cells. In addition, the cellular nicotinamide adenine dinucleotide autofluorescence enables in vivo imaging and counting of white blood cells (neutrophils).