ZFIN ID: ZDB-PUB-120702-56
Analysis of transcriptional codes for zebrafish dopaminergic neurons reveals essential functions of Arx and Isl1 in prethalamic dopaminergic neuron development
Filippi, A., Jainok, C., and Driever, W.
Date: 2012
Source: Developmental Biology 369(1): 133-149 (Journal)
Registered Authors: Driever, Wolfgang, Filippi, Alida, Jainok, Chamaiphorn
Keywords: dopaminergic neuron, transcription factors, zebrafish, diencephalon, telencephalon, neuronal specification
MeSH Terms: Animals; Body Patterning/drug effects; Body Patterning/genetics; Catecholamines/metabolism; Dopaminergic Neurons/cytology (all 34) expand
PubMed: 22728160 Full text @ Dev. Biol.
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ABSTRACT

Distinct groups of dopaminergic neurons develop at defined anatomical sites in the brain to modulate function of a large diversity of local and far-ranging circuits. However, the molecular identity as judged from transcription factor expression has not been determined for all dopaminergic groups. Here, we analyze regional expression of transcription factors in the larval zebrafish brain to determine co-expression with the Tyrosine hydroxylase marker in dopaminergic neurons. We define sets of transcription factors that clearly identify each dopaminergic group. These data confirm postulated relations to dopaminergic groups defined for mammalian systems. We focus our functional analysis on prethalamic dopaminergic neurons, which co-express the transcription factors Arx and Isl1. Morpholino-based knockdown reveals that both Arx and Isl1 are strictly required for prethalamic dopaminergic neuron development and appear to act in parallel. We further show that Arx contributes to patterning in the prethalamic region, while Isl1 is required for differentiation of prethalamic dopaminergic neurons.

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