PUBLICATION
VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells
- Authors
- Li, D., Li, X.P., Wang, H.X., Shen, Q.Y., Li, X.P., Wen, L., Qin, X.J., Jia, Q.L., Kung, H.F., and Peng, Y.
- ID
- ZDB-PUB-120702-26
- Date
- 2012
- Source
- Oncology reports 28(3): 937-942 (Journal)
- Registered Authors
- Li, Xiang, Peng, Ying
- Keywords
- none
- MeSH Terms
-
- Alkaline Phosphatase/metabolism
- Animals
- Cell Line, Tumor
- Cell Proliferation
- Cell Survival
- Embryo, Nonmammalian
- Gene Expression
- Glioma/blood supply*
- Glioma/pathology
- Humans
- Neoplasm Transplantation
- Neovascularization, Pathologic/enzymology
- Neovascularization, Pathologic/metabolism*
- Neovascularization, Pathologic/pathology
- Transplantation, Heterologous
- Vascular Endothelial Growth Factor A/genetics
- Vascular Endothelial Growth Factor A/physiology*
- Vascular Endothelial Growth Factor Receptor-2/genetics
- Vascular Endothelial Growth Factor Receptor-2/metabolism
- Zebrafish*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 22711017 Full text @ Oncol. Rep.
Citation
Li, D., Li, X.P., Wang, H.X., Shen, Q.Y., Li, X.P., Wen, L., Qin, X.J., Jia, Q.L., Kung, H.F., and Peng, Y. (2012) VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells. Oncology reports. 28(3):937-942.
Abstract
Zebrafish (Danio rerio) is becoming an increasingly popular vertebrate cancer model. In this study, we established a xenotransplanted zebrafish embryo glioma model to further investigate the molecular mechanisms of tumor angiogenesis. We find that the glioma cell line U87 can survive, proliferate and induce additional SIV branches in zebrafish embryos. In addition, by the means of in situ hybridization and quantitive RT-PCR analyses we find that the transplanted U87 cells can induce the ectopic zebrafish vascular endothelial growth factor A (VEGF A) and its receptor VEGFR2/KDR mRNA expression and increase their expression levels, resulting in additional SIV branches.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping