PUBLICATION

VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells

Authors
Li, D., Li, X.P., Wang, H.X., Shen, Q.Y., Li, X.P., Wen, L., Qin, X.J., Jia, Q.L., Kung, H.F., and Peng, Y.
ID
ZDB-PUB-120702-26
Date
2012
Source
Oncology reports   28(3): 937-942 (Journal)
Registered Authors
Li, Xiang, Peng, Ying
Keywords
none
MeSH Terms
  • Alkaline Phosphatase/metabolism
  • Neovascularization, Pathologic/enzymology
  • Neovascularization, Pathologic/metabolism*
  • Neovascularization, Pathologic/pathology
  • Cell Proliferation
  • Cell Line, Tumor
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Glioma/blood supply*
  • Glioma/pathology
  • Gene Expression
  • Neoplasm Transplantation
  • Transplantation, Heterologous
  • Vascular Endothelial Growth Factor Receptor-2/genetics
  • Vascular Endothelial Growth Factor Receptor-2/metabolism
  • Vascular Endothelial Growth Factor A/genetics
  • Vascular Endothelial Growth Factor A/physiology*
  • Cell Survival
  • Humans
  • Animals
  • Embryo, Nonmammalian
  • Zebrafish*
(all 22)
PubMed
22711017 Full text @ Oncol. Rep.
Abstract

Zebrafish (Danio rerio) is becoming an increasingly popular vertebrate cancer model. In this study, we established a xenotransplanted zebrafish embryo glioma model to further investigate the molecular mechanisms of tumor angiogenesis. We find that the glioma cell line U87 can survive, proliferate and induce additional SIV branches in zebrafish embryos. In addition, by the means of in situ hybridization and quantitive RT-PCR analyses we find that the transplanted U87 cells can induce the ectopic zebrafish vascular endothelial growth factor A (VEGF A) and its receptor VEGFR2/KDR mRNA expression and increase their expression levels, resulting in additional SIV branches.

Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Li, D., Li, X.P., Wang, H.X., Shen, Q.Y., Li, X.P., Wen, L., Qin, X.J., Jia, Q.L., Kung, H.F., and Peng, Y. (2012) VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells. Oncology reports. 28(3):937-942.