Diversification of IFNγ-inducible CXCb chemokines in cyprinid fish
- Authors
- van der Aa, L.M., Chadzinska, M., Derks, W., Scheer, M., Levraud, J.P., Boudinot, P., and Lidy Verburg-van Kemenade, B.M.
- ID
- ZDB-PUB-120702-17
- Date
- 2012
- Source
- Developmental and comparative immunology 38(2): 243-253 (Journal)
- Registered Authors
- Levraud, Jean-Pierre
- Keywords
- CXCb chemokines, cybrinids, promoter analysis, leukocyte stimulation
- MeSH Terms
-
- Animals
- Carps/immunology*
- Carps/metabolism
- Chemokines, CXC/genetics*
- Cloning, Molecular
- Evolution, Molecular
- Interferon-gamma/immunology
- Interferon-gamma/metabolism*
- Leukocytes/immunology
- Leukocytes/metabolism
- Lipopolysaccharides/metabolism
- Peritoneum/cytology
- Peritoneum/immunology
- Peritonitis/immunology
- Promoter Regions, Genetic
- Transcriptome
- Zebrafish/genetics
- Zebrafish/immunology
- Zymosan
- PubMed
- 22705555 Full text @ Dev. Comp. Immunol.
We earlier identified two CXCL8-like lineages in cyprinid fish, which are functional homologues of the mammalian CXCL8, but with diverged functions. We here investigated whether the carp IFN-γ-inducible CXCb gene, related to the mammalian CXCL9, -10 and -11 chemokines, was subject to a similar diversification. On the zebrafish genome, a cluster of seven CXCb genes was found on chromosome five. Analysis of the promoter of the zebrafish CXCb genes suggests a partially shared, but differential induction. A second CXCb gene, CXCb2, was identified in common carp by homology cloning. CXCb2 is constitutively expressed in immune-related tissues, predominantly in head kidney lymphocytes/monocytes. Interestingly, an induction of CXCb2 gene expression with recombinant carp IFN-γ2 and LPS was observed in macrophages and granulocytes. Finally, difference in sensitivity to LPS, and kinetics of CXCb1 and CXCb2 gene expression during zymosan-induced peritonitis, was observed. These results indicate a functional diversification for cyprinid CXCb chemokines, with functional homology to mammalian CXCL9–11.