PUBLICATION

Diversification of IFNγ-inducible CXCb chemokines in cyprinid fish

Authors
van der Aa, L.M., Chadzinska, M., Derks, W., Scheer, M., Levraud, J.P., Boudinot, P., and Lidy Verburg-van Kemenade, B.M.
ID
ZDB-PUB-120702-17
Date
2012
Source
Developmental and comparative immunology   38(2): 243-253 (Journal)
Registered Authors
Levraud, Jean-Pierre
Keywords
CXCb chemokines, cybrinids, promoter analysis, leukocyte stimulation
MeSH Terms
  • Animals
  • Carps/immunology*
  • Carps/metabolism
  • Chemokines, CXC/genetics*
  • Cloning, Molecular
  • Evolution, Molecular
  • Interferon-gamma/immunology
  • Interferon-gamma/metabolism*
  • Leukocytes/immunology
  • Leukocytes/metabolism
  • Lipopolysaccharides/metabolism
  • Peritoneum/cytology
  • Peritoneum/immunology
  • Peritonitis/immunology
  • Promoter Regions, Genetic
  • Transcriptome
  • Zebrafish/genetics
  • Zebrafish/immunology
  • Zymosan
PubMed
22705555 Full text @ Dev. Comp. Immunol.
Abstract

We earlier identified two CXCL8-like lineages in cyprinid fish, which are functional homologues of the mammalian CXCL8, but with diverged functions. We here investigated whether the carp IFN-γ-inducible CXCb gene, related to the mammalian CXCL9, -10 and -11 chemokines, was subject to a similar diversification. On the zebrafish genome, a cluster of seven CXCb genes was found on chromosome five. Analysis of the promoter of the zebrafish CXCb genes suggests a partially shared, but differential induction. A second CXCb gene, CXCb2, was identified in common carp by homology cloning. CXCb2 is constitutively expressed in immune-related tissues, predominantly in head kidney lymphocytes/monocytes. Interestingly, an induction of CXCb2 gene expression with recombinant carp IFN-γ2 and LPS was observed in macrophages and granulocytes. Finally, difference in sensitivity to LPS, and kinetics of CXCb1 and CXCb2 gene expression during zymosan-induced peritonitis, was observed. These results indicate a functional diversification for cyprinid CXCb chemokines, with functional homology to mammalian CXCL9–11.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping