PUBLICATION

Spatiotemporal photolabeling of neutrophil trafficking during inflammation in live zebrafish

Authors
Yoo, S.K., and Huttenlocher, A.
ID
ZDB-PUB-120611-10
Date
2011
Source
Journal of Leukocyte Biology   89(5): 661-667 (Journal)
Registered Authors
Huttenlocher, Anna
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Blotting, Western
  • Cell Movement*
  • Disease Models, Animal*
  • Embryo, Nonmammalian/metabolism
  • Embryo, Nonmammalian/radiation effects
  • Image Processing, Computer-Assisted
  • Inflammation/metabolism
  • Inflammation/pathology*
  • Light*
  • Luminescent Proteins/chemistry*
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • Neutrophils/metabolism*
  • Neutrophils/pathology
  • RNA, Messenger/genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Wound Healing
  • Zebrafish/growth & development
  • Zebrafish/metabolism*
PubMed
21248150 Full text @ J. Leukoc. Biol.
Abstract

How neutrophils traffic during inflammation in vivo remains elusive. To visualize the origin and fate of neutrophils during induction and resolution of inflammation, we established a genetically encoded photolabeling system by generating transgenic zebrafish that express a photoconvertible fluorescent reporter Dendra2 in neutrophils. Spatiotemporal photolabeling of neutrophils in vivo demonstrates that they emerge from the hematopoietic tissue in close proximity to injured tissue and repeat forward and reverse migration between the wound and the vasculature. Subsequently, neutrophils disperse throughout the body as wound-healing proceeds, contributing to local resolution at injured tissue and systemic dissemination of wound-sensitized neutrophils. Tissue damage also alters the fate of neutrophils in the caudal hematopoietic tissue and promotes caudorostral mobilization of neutrophils via the circulation to the cephalic mesenchyme. This work provides new insight into neutrophil behaviors during inflammation and resolution within a multicellular organism.

Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes