ZFIN ID: ZDB-PUB-120529-5
Kidins220/ARMS interacts with Pdzrn3, a protein containing multiple binding domains
Andreazzoli, M., Gestri, G., Landi, E., D'Orsi, B., Barilari, M., Iervolino, A., Vitiello, M., Wilson, S.W., and Dente, L.
Date: 2012
Source: Biochimie   94(9): 2054-2057 (Journal)
Registered Authors: Andreazzoli, Massimiliano, Gestri, Gaia, Wilson, Steve
Keywords: binding motif, neural development, PDZ-domain, protein-protein interaction
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Carrier Proteins/chemistry*
  • Carrier Proteins/metabolism*
  • Computational Biology
  • HEK293 Cells
  • Humans
  • Membrane Proteins/chemistry
  • Membrane Proteins/metabolism*
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins/chemistry
  • Nerve Tissue Proteins/metabolism*
  • PC12 Cells
  • Protein Binding
  • Protein Structure, Tertiary
  • Rats
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/metabolism
PubMed: 22609016 Full text @ Biochimie

We report the identification of a novel partner of Kidins220/ARMS (Kinase D-interacting substrate of 220 kDa/Ankyrin Repeat-rich Membrane Spanning) an adaptor of neurotrophin receptors playing crucial roles during neurogenesis. Screening a phage display library of brain cDNA products we found that D. rerio Pdzrn3, a protein containing RING-finger and PDZ-domains, interacts with Kidins220/ARMS through its first PDZ-domain. Both zebrafish proteins share high homology with the corresponding mammalian proteins and both genes are developmentally expressed in neural districts where early neurogenesis occurs. The interaction was also confirmed by biochemical assays and by co-localization at the tips of growing neurites of PC12 cells induced with nerve growth factor.