PUBLICATION
An optimized two-finger archive for ZFN-mediated gene targeting
- Authors
- Gupta, A., Christensen, R.G., Rayla, A.L., Lakshmanan, A., Stormo, G.D., and Wolfe, S.A.
- ID
- ZDB-PUB-120503-7
- Date
- 2012
- Source
- Nature Methods 9(6): 588-590 (Journal)
- Registered Authors
- Gupta, Ankit, Lakshmanan, Abirami, Wolfe, Scot A.
- Keywords
- none
- MeSH Terms
-
- Animals
- Catalytic Domain
- DNA Breaks, Double-Stranded
- Endodeoxyribonucleases/genetics
- Endodeoxyribonucleases/metabolism
- Gene Targeting/methods*
- Zebrafish
- Zinc Fingers/genetics*
- PubMed
- 22543349 Full text @ Nat. Methods
Citation
Gupta, A., Christensen, R.G., Rayla, A.L., Lakshmanan, A., Stormo, G.D., and Wolfe, S.A. (2012) An optimized two-finger archive for ZFN-mediated gene targeting. Nature Methods. 9(6):588-590.
Abstract
The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping