PUBLICATION
In silico analysis of the regulatory region of the Yellowtail Kingfish and Zebrafish Kiss and Kiss receptor genes
- Authors
- Nocillado, J.N., Mechaly, A.S., and Elizur, A.
- ID
- ZDB-PUB-120426-5
- Date
- 2013
- Source
- Fish physiology and biochemistry 39(1): 59-63 (Journal)
- Registered Authors
- Elizur, Abigail, Mechaly, Alejandro
- Keywords
- Kiss, GPR54, kisspeptin, puberty
- MeSH Terms
-
- Animals
- Base Sequence
- Cloning, Molecular
- Computational Biology
- DNA Primers/genetics
- Kisspeptins/genetics*
- Molecular Sequence Data
- Perciformes/genetics*
- Polymerase Chain Reaction
- Receptors, G-Protein-Coupled/genetics*
- Regulatory Elements, Transcriptional/genetics*
- Sequence Analysis, DNA
- Zebrafish/genetics*
- PubMed
- 22527613 Full text @ Fish Physiol. Biochem.
Citation
Nocillado, J.N., Mechaly, A.S., and Elizur, A. (2013) In silico analysis of the regulatory region of the Yellowtail Kingfish and Zebrafish Kiss and Kiss receptor genes. Fish physiology and biochemistry. 39(1):59-63.
Abstract
We have cloned and analysed the partial putative promoter sequences of the Yellowtail Kingfish (Seriola lalandi) Kiss2 and Kiss2r genes (380 and 420 bp, respectively). We obtained in silico 1.5 kb of the zebrafish (Danio rerio) Kiss1, Kiss2, Kiss1r and zfKiss2r sequences upstream of the putative transcriptional initiation site. Bioinformatic analysis revealed promoter regulatory elements including AP-1, Sp1, GR, ER, PR, AR, GATA-1, TTF-1, YY1 and C/EBP. These regulatory elements may mediate novel roles of the Kiss genes and their receptors in addition to their established role in reproductive function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping