Rodrigues, F.S., Doughton, G., Yang, B., and Kelsh, R.N. (2012) A novel transgenic line using the Cre-lox system to allow permanent lineage labelling of the zebrafish neural crest. Genesis (New York, N.Y. : 2000). 50(10):750-757.
Accurate lineage tracing is crucial to understanding of developmental and stem cell biology, but is particularly challenging for transient and highly dispersive cell-types like the neural crest (NC). We report here a new zebrafish transgenic line Tg(-4725sox10:Cre)ba74. This line expresses Cre under the control of a well-characterised portion of the sox10 promoter and, by crossing to a floxed reporter line, we show here that expression in this line is consistent with those described for GFP reporter lines using the same promoter. Reporter expression is readily detected in patterns consistent with the early expression domains. Thus we see all major groups (pigment, neural and skeletal) of NC-derived cell-types, as well as cell-types derived from the known non-NC sites of sox10 expression, including otic epithelium and oligodendrocytes. This line provides an invaluable tool for the further study of zebrafish NC development and NC-derived stem cells, as well as that of the otic vesicle and oligodendrocytes.