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ZIRC
ZFIN ID: ZDB-PUB-120424-29
A novel transgenic line using the Cre-lox system to allow permanent lineage labelling of the zebrafish neural crest
Rodrigues, F.S., Doughton, G., Yang, B., and Kelsh, R.N.
Date: 2012
Source: Genesis (New York, N.Y. : 2000) 50(10): 750-757 (Journal)
Registered Authors: Kelsh, Robert
Keywords: danio rerio, otic epithelium, pigment cells, melanocyte, xanthophore, iridophore, PNS, enteric nervous system, oligodendrocytes, Schwann cells, sox10
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/embryology
  • Animals, Genetically Modified/genetics*
  • Cell Lineage*
  • Genes, Reporter
  • Integrases/genetics*
  • Integrases/metabolism
  • Neural Crest/cytology*
  • Neural Crest/growth & development
  • Neural Stem Cells/cytology*
  • Promoter Regions, Genetic
  • SOXE Transcription Factors/genetics
  • SOXE Transcription Factors/metabolism
  • Transcription, Genetic
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 22522888 Full text @ Genesis
ABSTRACT

Accurate lineage tracing is crucial to understanding of developmental and stem cell biology, but is particularly challenging for transient and highly dispersive cell-types like the neural crest (NC). We report here a new zebrafish transgenic line Tg(-4725sox10:Cre)ba74. This line expresses Cre under the control of a well-characterised portion of the sox10 promoter and, by crossing to a floxed reporter line, we show here that expression in this line is consistent with those described for GFP reporter lines using the same promoter. Reporter expression is readily detected in patterns consistent with the early expression domains. Thus we see all major groups (pigment, neural and skeletal) of NC-derived cell-types, as well as cell-types derived from the known non-NC sites of sox10 expression, including otic epithelium and oligodendrocytes. This line provides an invaluable tool for the further study of zebrafish NC development and NC-derived stem cells, as well as that of the otic vesicle and oligodendrocytes.

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