PUBLICATION

Edwardsiella tarda flagellar protein FlgD: A protective immunogen against edwardsiellosis

Authors
Zhang, M., Wu, H., Li, X., Yang, M., Chen, T., Wang, Q., Liu, Q., and Zhang, Y.
ID
ZDB-PUB-120424-22
Date
2012
Source
Vaccine   30(26): 3849-3856 (Journal)
Registered Authors
Liu, Qin
Keywords
Edwardsiella tarda, flagellar protein, FlgD, protective antigen
MeSH Terms
  • Survival Analysis
  • Molecular Sequence Data
  • Animals
  • Flatfishes
  • Gene Expression
  • Flagellin/genetics
  • Flagellin/immunology*
  • Recombinant Proteins/genetics
  • Recombinant Proteins/immunology
  • Enterobacteriaceae Infections/prevention & control
  • Enterobacteriaceae Infections/veterinary*
  • Zebrafish
  • Sequence Analysis, DNA
  • Bacterial Vaccines/administration & dosage*
  • Bacterial Vaccines/immunology*
  • DNA, Bacterial/chemistry
  • DNA, Bacterial/genetics
  • Escherichia coli/genetics
  • Disease Models, Animal
  • Edwardsiella tarda/genetics
  • Edwardsiella tarda/immunology*
PubMed
22521284 Full text @ Vaccine
Abstract

Edwardsiella tarda is a gram-negative bacterium and a causative agent of edwardsiellosis, resulting to severe loss of the aquaculture industry. In this study, based on the reverse vaccinology, sixteen flagellar proteins were selected from highly pathogenic E. tarda EIB202 genome information and in silico analyzed as potential vaccine candidates. Among them, ten recombinant proteins were highly expressed in Escherichia coli and successfully purified. The immunoprotective potentials of these purified recombinant proteins were evaluated in zebrafish model. And recombinant FlgD and FliD were found to lead to a high relative percent survival (RPS, about 70%) against E. tarda EIB202. Furthermore, FlgD required in flagellum hook assembly brought about the similar immune protection in turbot. The immune responses of zebrafish and turbot to recombinant FlgD were also investigated, and the results indicated that its high protection was mainly involved in cellular mediated immune response, corresponding to the intracellular pathogenicity of E. tarda.

Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping