Identification and expression of a novel member of Ly-6 superfamily in zebrafish Denio rerio
- Authors
- Ji, D., Liu, P., Wang, F., Zhang, S., and Li, H.
- ID
- ZDB-PUB-120316-3
- Date
- 2012
- Source
- Development genes and evolution 222(2): 119-124 (Journal)
- Registered Authors
- Li, Hongyan
- Keywords
- zebrafish, Ly-6, LU, epidermis
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Humans
- Lymphocyte Antigen 96/chemistry
- Lymphocyte Antigen 96/genetics
- Lymphocyte Antigen 96/immunology
- Lymphocyte Antigen 96/isolation & purification
- Molecular Sequence Data
- Sequence Alignment
- Zebrafish/embryology
- Zebrafish/metabolism*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/immunology
- Zebrafish Proteins/isolation & purification
- PubMed
- 22415300 Full text @ Dev. Genes Evol.
Ly-6 superfamily members are present many metazoans and are divided into two groups: secreted proteins and glycosylphosphatidyl inositol (GPI)-anchored membrane proteins. They both contain one or more conserved domain identified as Ly-6/uPAR (LU) domain and play key roles in cellular adhesion and signaling. Here, we identify a novel member, lymphocyte antigen-6 epidermis (lye), of Ly-6 superfamily zebrafish. In silico analyses revealed that lye codes for a predicted GPI-anchored membrane protein containing a conserved LU domain and 10 position-specific conserved cysteines typical of known Ly-6 proteins. Whole mount situ hybridization showed that lye is predominantly expressed in epidermis. We thus named the gene lye, highlighting it is expressed in epidermis. Lye exhibits a dynamic expression pattern during development, which is initially expressed in enveloping layer at gastrula stage, then expressed in epidermis at later stages. It is also expressed in olfactory placode at 24 h post-fertilization. Subsequently, epidermal expression of lye becomes weaker gradually, whereas the expression in pharyngeal arch and pectoral fin increases at 2 and 3 days post-fertilization. Our study lays a foundation for further investigation of lye roles in early developmental stages.