Role of lbx2 in the noncanonical Wnt signaling pathway for convergence and extension movements and hypaxial myogenesis in zebrafish
- Authors
- Lou, Q., He, J., Hu, L., and Yin, Z.
- ID
- ZDB-PUB-120314-20
- Date
- 2012
- Source
- Biochimica et biophysica acta. Molecular cell research 1823(5): 1024-1032 (Journal)
- Registered Authors
- He, Jiangyan, Yin, Zhan
- Keywords
- zebrafish, lbx2, noncanonical wnt, gastrulation
- MeSH Terms
-
- Enzyme Activation/drug effects
- Mesoderm/cytology
- Animals
- Gene Expression Regulation, Developmental/drug effects
- Wnt Proteins/genetics
- Wnt Proteins/metabolism
- Morpholinos/pharmacology
- Repressor Proteins/genetics
- Repressor Proteins/metabolism*
- Muscles/cytology
- Muscles/embryology
- Mice
- Gastrulation/drug effects
- Gastrulation/genetics
- Stem Cells/cytology
- Stem Cells/drug effects
- Stem Cells/metabolism
- Wnt Signaling Pathway*/drug effects
- Wnt Signaling Pathway*/genetics
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/enzymology
- Body Patterning*/drug effects
- Body Patterning*/genetics
- Animal Fins/cytology
- Animal Fins/drug effects
- Animal Fins/embryology
- Zebrafish/embryology*
- Zebrafish/genetics
- Muscle Development*/genetics
- rhoA GTP-Binding Protein/metabolism
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Cell Movement*/drug effects
- Cell Movement*/genetics
- PubMed
- 22406073 Full text @ BBA Molecular Cell Research
It has been suggested that mouse lbx1 is essential for directing hypaxial myogenic precursor cell migration. In zebrafish, the expression of lbx1a, lbx1b, and lbx2 has been observed in pectoral fin buds. It has also been shown that knocking down endogenous lbx2 in zebrafish embryos diminishes myoD expression in the pectoral fin bud. However, downstream lbxs signals remain largely unexplored. Here, we describe a previously unknown function of zebrafish lbx2 (lbx2) during convergent extension (CE) movements. The abrogation of the lbx2 function by two non-overlapping morpholino oligonucleotides (MOs) resulted in the defective convergence and extension movements in morphants during gastrulation. Our transplantation studies further demonstrated that the overexpression of lbx2 autonomously promotes CE movements. Expression of wnt5b is significantly reduced in lbx2 morphants. We have demonstrated that application of the wnt5b MO, a dominant-negative form of disheveled (Dvl) and a chemical inhibitor of Rho-associated kinase Y27632 in zebrafish embryos have effects reminiscent that are of the CE and hypaxial myogenesis defects observed in lbx2 morphants. Moreover, the CE and hypaxial mesoderm defects seen in lbx2 morphants can be rescued by co-injection with wnt5b or RhoA mRNA. However, this reduced level of active RhoA and hypaxial myogenesis defects in the embryos injected with the dominant-negative form of Dvl mRNA cannot be effectively restored by co-injection with lbx2 mRNA. Our results suggest that the key noncanonical Wnt signaling components Wnt5, Dvl, and RhoA are downstream effectors involved in the regulative roles of lbx2 in CE movement and hypaxial myogenesis during zebrafish embryogenesis.