Mutations in axonemal dynein assembly factor DNAAF3 cause primary ciliary dyskinesia
- Authors
- Mitchison, H.M., Schmidts, M., Loges, N.T., Freshour, J., Dritsoula, A., Hirst, R.A., O'Callaghan, C., Blau, H., Al Dabbagh, M., Olbrich, H., Beales, P.L., Yagi, T., Mussaffi, H., Chung, E.M., Omran, H., and Mitchell, D.R.
- ID
- ZDB-PUB-120306-22
- Date
- 2012
- Source
- Nature Genetics 44(4): 381-389 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Chlamydomonas reinhardtii/genetics
- Axonemal Dyneins/biosynthesis*
- Female
- Sequence Analysis, DNA
- Microtubule-Associated Proteins/chemistry
- Microtubule-Associated Proteins/genetics*
- Phenotype
- Amino Acid Sequence
- Bacterial Proteins/chemistry
- Bacterial Proteins/genetics
- Bacterial Proteins/metabolism
- Zebrafish/genetics
- Base Sequence
- Humans
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Cytoplasm/genetics
- Molecular Sequence Data
- Mutation
- Polymorphism, Single Nucleotide
- Male
- Situs Inversus/genetics
- Pedigree
- Cilia/genetics
- Kartagener Syndrome/genetics*
- Animals
- Sequence Alignment
- PubMed
- 22387996 Full text @ Nat. Genet.
Primary ciliary dyskinesia most often arises from loss of the dynein motors that power ciliary beating. Here we show that DNAAF3 (also known as PF22), a previously uncharacterized protein, is essential for the preassembly of dyneins into complexes before their transport into cilia. We identified loss-of-function mutations in the human DNAAF3 gene in individuals from families with situs inversus and defects in the assembly of inner and outer dynein arms. Knockdown of dnaaf3 in zebrafish likewise disrupts dynein arm assembly and ciliary motility, causing primary ciliary dyskinesia phenotypes that include hydrocephalus and laterality malformations. Chlamydomonas reinhardtii PF22 is exclusively cytoplasmic, and a PF22-null mutant cannot assemble any outer and some inner dynein arms. Altered abundance of dynein subunits in mutant cytoplasm suggests that DNAAF3 (PF22) acts at a similar stage as other preassembly proteins, for example, DNAAF2 (also known as PF13 or KTU) and DNAAF1 (also known as ODA7 or LRRC50), in the dynein preassembly pathway. These results support the existence of a conserved, multistep pathway for the cytoplasmic formation of assembly competent ciliary dynein complexes.