PUBLICATION

"Vacuum-assisted staining": a simple and efficient method for screening in Drosophila

Authors
Berns, N., Woichansky, I., Kraft, N., Hüsken, U., Carl, M., and Riechmann, V.
ID
ZDB-PUB-120301-15
Date
2012
Source
Development genes and evolution   222(2): 113-118 (Journal)
Registered Authors
Carl, Matthias
Keywords
Drosophila, screening, in vivo RNAi, follicular epithelium, imaginal disc, larval brain, zebrafish
MeSH Terms
  • Animals
  • Drosophila/anatomy & histology
  • Drosophila/embryology*
  • Larva/anatomy & histology
  • Staining and Labeling/economics
  • Staining and Labeling/methods*
  • Zebrafish/embryology
PubMed
22367217 Full text @ Dev. Genes Evol.
Abstract

The constantly growing number of genetic tools rapidly increases possibilities for various screens in different model organisms and calls for new methods facilitating screen performance. In particular, screening procedures involving fixation and staining of samples are difficult to perform at a genome-wide scale. The time-consuming task to generate these samples makes such screens less attractive. Here, we describe the use of multi-well filter plates for high throughput labellings of different Drosophila organs and zebrafish embryos. Our inexpensive vacuum-assisted staining protocol minimises the risk of sample loss, reduces the amount of staining reagents and drastically decreases labour and repetitive work. The simple handling of the system and the commercial availability of its components makes this method easily applicable to every laboratory.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping