ZFIN ID: ZDB-PUB-120223-36
Identification of the Receptors for Prolactin-Releasing Peptide (PrRP) and Carassius RFamide Peptide (C-RFa) in Chickens
Wang, Y., Wang, C.Y., Wu, Y., Huang, G., Li, J., and Leung, F.C.
Date: 2012
Source: Endocrinology   153(4): 1861-1874 (Journal)
Registered Authors:
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Brain/metabolism*
  • Chick Embryo
  • Chickens/metabolism*
  • Chromosome Mapping
  • Cyclic AMP-Dependent Protein Kinases/physiology
  • DNA, Complementary/metabolism
  • Gene Expression Regulation
  • Humans
  • Mice
  • Molecular Sequence Data
  • Neuropeptides/genetics*
  • Neuropeptides/metabolism*
  • Rats
  • Receptors, Neuropeptide/genetics*
  • Receptors, Neuropeptide/metabolism*
  • Signal Transduction/physiology
  • Xenopus
  • Zebrafish
PubMed: 22355069 Full text @ Endocrinology

Prolactin-releasing peptide (PrRP) and its structurally related peptide, Carassius Arg-Phe-amide peptide (C-RFa), have been reported to play similar roles in regulating food intake and pituitary functions in vertebrates. However, the identity, functionality, and expression of the receptor(s) for PrRP and C-RFa remain largely unknown in nonmammalian vertebrates, including birds. In this study, three receptors homologous to mammalian PrRP receptor (PrRPR), named cPrRPR1, cPrRPR2, and cC-RFaR, respectively, were cloned from chicken brain by RT-PCR. Using a pGL3-NFAT-RE-luciferase reporter system, we demonstrated that cPrRPR1 and cPrRPR2 expressed in Chinese hamster ovarian cells could be activated by cPrRP20 and cC-RFa20 potently, whereas cC-RFaR could only be activated effectively by cC-RFa20 (EC50, 0.11 nm), indicating that cPrRPR1 and cPrRPR2 can function as common receptors for PrRP and C-RFa, whereas cC-RFaR is a receptor specific to C-RFa. Using a pGL3-CRE-luciferase reporter system, cPrRPR1, cPrRPR2, and cC-RFaR expressed in Chinese hamster ovarian cells were also shown to activate intracellular protein kinase A signaling pathway upon cC-RFa20 treatment (100 nm). Moreover, RT-PCR assay revealed that cPrRPR1, cPrRPR2, and cC-RFaR were widely expressed in most adult chicken tissues examined, including various regions of brain. These findings, together with evidence of PrRP and C-RFa encoded by separate genes in chicken, Xenopus, and zebrafish, and the differential expression of PrRP and C-RFa genes in chicken tissues, strongly suggest that PrRP and C-RFa may play similar yet distinctive roles in nonmammalian vertebrates, including chicken, and their actions are mediated by common receptor(s) or a specific C-RFa receptor.