PUBLICATION

Techniques for the in vivo assessment of cardio-renal function in zebrafish (Danio rerio) larvae

Authors
Rider, S.A., Tucker, C.S., Del-Pozo, J., Rose, K.N., MacRae, C.A., Bailey, M.A., and Mullins, J.J.
ID
ZDB-PUB-120216-8
Date
2012
Source
The Journal of physiology   590(8): 1803-1809 (Journal)
Registered Authors
MacRae, Calum A.
Keywords
renal function, zebrafish, inulin
MeSH Terms
  • Animals
  • Cardiovascular Physiological Phenomena
  • Heart/physiology*
  • Inulin/metabolism
  • Kidney Function Tests
  • Kidney Glomerulus/physiology*
  • Larva
  • Models, Animal*
  • Urinary Tract Physiological Phenomena
  • Zebrafish/physiology*
PubMed
22331420 Full text @ J. Physiol.
Abstract
Zebrafish, a well-established vertebrate model, offer unique advantages for assessing renal function and physiology. Assays determining renal glomerular function based on cardiovascular erythrocyte flow and reduction of injected FITC-inulin were developed; each validated using the nephrotoxin gentamicin. Bland-Atlman analysis showed a strong association between measurements of the rate of inulin excretion and that of fluorescent reduction from the arterial vasculature. Reduced renal clearance of inulin, resulting from gentamicin or NaCl loading, was concurrent with reduced erythrocyte velocity, and yolk sac and pericardium oedema. These techniques, assessing pronephric function, highlight the potential for in-vivo physiological study in this genetically tractable model.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes