pitx2 Deficiency Results in Abnormal Ocular and Craniofacial Development in Zebrafish
- Authors
- Liu, Y., and Semina, E.V.
- ID
- ZDB-PUB-120207-8
- Date
- 2012
- Source
- PLoS One 7(1): e30896 (Journal)
- Registered Authors
- Semina, Elena
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- Biomarkers/metabolism
- Craniofacial Abnormalities/embryology*
- Craniofacial Abnormalities/metabolism
- Craniofacial Abnormalities/pathology
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Embryonic Development/drug effects
- Exons/genetics
- Eye Abnormalities/embryology*
- Eye Abnormalities/pathology
- Gene Expression Regulation, Developmental/drug effects
- Gene Knockdown Techniques
- Homeodomain Proteins/metabolism
- Humans
- In Situ Hybridization
- Molecular Sequence Data
- Morpholinos/pharmacology
- Phenotype
- Protein Biosynthesis/drug effects
- RNA Splicing/drug effects
- RNA Splicing/genetics
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Transcription Factors/deficiency*
- Transcription Factors/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/deficiency*
- Zebrafish Proteins/metabolism
- PubMed
- 22303467 Full text @ PLoS One
Human PITX2 mutations are associated with Axenfeld-Rieger syndrome, an autosomal-dominant developmental disorder that involves ocular anterior segment defects, dental hypoplasia, craniofacial dysmorphism and umbilical abnormalities. Characterization of the PITX2 pathway and identification of the mechanisms underlying the anomalies associated with PITX2 deficiency is important for better understanding of normal development and disease; studies of pitx2 function in animal models can facilitate these analyses. A knockdown of pitx2 in zebrafish was generated using a morpholino that targeted all known alternative transcripts of the pitx2 gene; morphant embryos generated with the pitx2ex4/5 splicing-blocking oligomer produced abnormal transcripts predicted to encode truncated pitx2 proteins lacking the third (recognition) helix of the DNA-binding homeodomain. The morphological phenotype of pitx2ex4/5 morphants included small head and eyes, jaw abnormalities and pericardial edema; lethality was observed at ~6–8-dpf. Cartilage staining revealed a reduction in size and an abnormal shape/position of the elements of the mandibular and hyoid pharyngeal arches; the ceratobranchial arches were also decreased in size. Histological and marker analyses of the misshapen eyes of the pitx2ex4/5 morphants identified anterior segment dysgenesis and disordered hyaloid vasculature. In summary, we demonstrate that pitx2 is essential for proper eye and craniofacial development in zebrafish and, therefore, that PITX2/pitx2 function is conserved in vertebrates.