PUBLICATION
Ethanol Exposure Induces Upregulation of Specific MicroRNAs in Zebrafish Embryos
- Authors
- Soares, A.R., Pereira, P.M., Ferreira, V., Reverendo, M., Simões, J., Bezerra, A.R., Moura, G.R., and Santos, M.A.
- ID
- ZDB-PUB-120203-5
- Date
- 2012
- Source
- Toxicological sciences : an official journal of the Society of Toxicology 127(1): 18-28 (Journal)
- Registered Authors
- Santos, Manuel
- Keywords
- ethanol toxicity, microRNAs, zebrafish, apoptosis
- Datasets
- GEO:GSE32632
- MeSH Terms
-
- Abnormalities, Drug-Induced/etiology
- Abnormalities, Drug-Induced/genetics
- Abnormalities, Drug-Induced/metabolism
- Animals
- Apoptosis/drug effects
- Apoptosis/genetics
- Biomarkers/metabolism
- Cell Cycle/drug effects
- Cell Cycle/genetics
- Central Nervous System Depressants/toxicity*
- Embryo, Nonmammalian/abnormalities
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- Ethanol/toxicity*
- Gene Expression Regulation, Developmental/drug effects*
- MicroRNAs/drug effects*
- MicroRNAs/genetics
- Real-Time Polymerase Chain Reaction
- Up-Regulation/drug effects*
- Zebrafish
- PubMed
- 22298809 Full text @ Toxicol. Sci.
- CTD
- 22298809
Citation
Soares, A.R., Pereira, P.M., Ferreira, V., Reverendo, M., Simões, J., Bezerra, A.R., Moura, G.R., and Santos, M.A. (2012) Ethanol Exposure Induces Upregulation of Specific MicroRNAs in Zebrafish Embryos. Toxicological sciences : an official journal of the Society of Toxicology. 127(1):18-28.
Abstract
Prenatal exposure to ethanol leads to a myriad of developmental disorders known as fetal alcohol spectrum disorder, often
characterized by growth and mental retardation, central nervous system damage and specific craniofacial dysmorphic features.
The mechanisms of ethanol toxicity are not fully understood, but exposure during development affects the expression of several
genes involved in cell cycle control, apoptosis and transcriptional regulation. MicroRNAs (miRNAs) are implicated in some
of these processes however it is not yet clear if they are involved in ethanol-induced toxicity. In order to clarify this
question we have exposed zebrafish embryos to ethanol and evaluated whether a miRNA deregulation signature could be obtained.
Zebrafish embryos were exposed to 1% and 1.5% of ethanol from 4 hours post-fertilization (hpf) to 24hpf. The miRNA expression
profiles obtained reveal significant miRNA deregulation and show that both ethanol concentrations up-regulate miR-153a, miR-725,
miR-30d, let-7k, miR-100, miR-738 and miR-732. Putative gene targets of deregulated miRNAs are involved in cell cycle control,
apoptosis and transcription, which are the main processes affected by ethanol toxicity. The conservation of affected mechanisms
among vertebrates leads us to postulate that similar miRNA deregulation occurs in humans, highlighting a relevant role of
miRNAs in ethanol toxicology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping