ZFIN ID: ZDB-PUB-120127-5
Tol2 gene trap integrations in the zebrafish amyloid precursor protein genes appa and aplp2 reveal accumulation of secreted APP at the embryonic veins
Liao, H.K., Wang, Y., Watt, K.E., Wen, Q., Breitback, J., Kemmet, C.K., Clark, K.J., Ekker, S.C., Essner, J.J., and McGrail, M.
Date: 2012
Source: Developmental dynamics : an official publication of the American Association of Anatomists   241(2): 415-425 (Journal)
Registered Authors: Clark, Karl, Ekker, Stephen C., Essner, Jeffrey, McGrail, Maura
Keywords: Tol2 gene trap, endothelial cells, vein, vasculature, central nervous system
MeSH Terms:
  • Alleles
  • Amyloid beta-Protein Precursor/genetics
  • Amyloid beta-Protein Precursor/metabolism*
  • Amyloidogenic Proteins/genetics
  • Amyloidogenic Proteins/metabolism*
  • Animals
  • DNA Transposable Elements/genetics
  • Fluorescent Dyes/metabolism
  • Genetic Techniques
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • Mutagenesis, Insertional
  • Veins/embryology
  • Veins/metabolism
  • Zebrafish/embryology*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 22275008 Full text @ Dev. Dyn.

Background: The single spanning transmembrane amyloid precursor protein (APP) and its proteolytic product, amyloid-beta (Aβ) peptide, have been intensely studied due to their role in the pathogenesis of Alzheimer's disease. However, the biological role of the secreted ectodomain of APP, which is also generated by proteolytic cleavage, is less well understood. Here, we report Tol2 red fluorescent protein (RFP) transposon gene trap integrations in the zebrafish amyloid precursor protein a (appa) and amyloid precursor-like protein 2 (aplp2) genes. The transposon integrations are predicted to disrupt the appa and aplp2 genes to primarily produce secreted ectodomains of the corresponding proteins that are fused to RFP. Results: Our results indicate the Appa-RFP and Aplp2 fusion proteins are likely secreted from the central nervous system and accumulate in the embryonic veins independent of blood flow. Conclusions: The zebrafish appa and aplp2 transposon insertion alleles will be useful for investigating the biological role of the secreted form of APP.