PUBLICATION

Transcriptional signature of accessory cells in the lateral line, using the Tnk1bp1:EGFP transgenic zebrafish line

Authors
Behra, M., Gallardo, V.E., Bradsher, J., Torrado, A., Elkahloun, A., Idol, J., Sheehy, J., Zonies, S., Xu, L., Shaw, K.M., Satou, C., Higashijima, S.I., Weinstein, B.M., and Burgess, S.M.
ID
ZDB-PUB-120126-1
Date
2012
Source
BMC Developmental Biology   12(1): 6 (Journal)
Registered Authors
Behra, Martine, Burgess, Shawn, Gallardo, Viviana, Higashijima, Shin-ichi, Weinstein, Brant M., Xu, Lisha
Keywords
none
MeSH Terms
  • Recombinant Fusion Proteins/biosynthesis
  • Recombinant Fusion Proteins/genetics
  • Larva/cytology
  • Larva/metabolism
  • In Situ Hybridization
  • Oligonucleotide Array Sequence Analysis
  • Animals
  • Mutagenesis, Insertional
  • Molecular Sequence Data
  • Gene Expression
  • Olfactory Mucosa/metabolism
  • Animals, Genetically Modified*
  • Amino Acid Sequence
  • Genetic Markers
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Green Fluorescent Proteins/biosynthesis
  • Green Fluorescent Proteins/genetics*
  • Transcriptome*
  • Genes, Reporter
  • Real-Time Polymerase Chain Reaction
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Lateral Line System/cytology*
  • Lateral Line System/growth & development
  • Transcription, Genetic
  • Telomeric Repeat Binding Protein 1/chemistry
  • Telomeric Repeat Binding Protein 1/genetics*
  • Telomeric Repeat Binding Protein 1/metabolism
(all 30)
PubMed
22273551 Full text @ BMC Dev. Biol.
Abstract

Background

Because of the structural and molecular similarities between the two systems, the lateral line, a fish and amphibian specific sensory organ, has been widely used in zebrafish as a model to study the development/biology of neuroepithelia of the inner ear. Both organs have hair cells, which are the mechanoreceptor cells, and supporting cells providing other functions to the epithelium. In most vertebrates (excluding mammals), supporting cells comprise a pool of progenitors that replace damaged or dead hair cells. However, the lack of regenerative capacity in mammals is the single leading cause for acquired hearing disorders in humans.

Results

In an effort to understand the regenerative process of hair cells in fish, we characterized and cloned an egfp transgenic stable fish line that trapped tnks1bp1, a highly conserved gene that has been implicated in the maintenance of telomeres' length. We then used this Tg(tnks1bp1:EGFP) line in a FACsorting strategy combined with microarrays to identify new molecular markers for supporting cells.

Conclusions

We present a Tg(tnks1bp1:EGFP) stable transgenic line, which we used to establish a transcriptional profile of supporting cells in the zebrafish lateral line. Therefore we are providing a new set of markers specific for supporting cells as well as candidates for functional analysis of this important cell type. This will prove to be a valuable tool for the study of regeneration in the lateral line of zebrafish in particular and for regeneration of neuroepithelia in general.

Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
nns8TgTransgenic Insertion
    y229GtTransgenic Insertion
    1 - 2 of 2
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    Fish
    nns8Tg
    tnks1bp1y229Gt
    tnks1bp1y229Gt; nns8Tg
    WT
    1 - 4 of 4
    Show
    Antibodies
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    EGFPEFGEGFP
    TomatoEFGTomato
    1 - 2 of 2
    Show
    Mapping
    No data available
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    Citation
    Behra, M., Gallardo, V.E., Bradsher, J., Torrado, A., Elkahloun, A., Idol, J., Sheehy, J., Zonies, S., Xu, L., Shaw, K.M., Satou, C., Higashijima, S.I., Weinstein, B.M., and Burgess, S.M. (2012) Transcriptional signature of accessory cells in the lateral line, using the Tnk1bp1:EGFP transgenic zebrafish line. BMC Developmental Biology. 12(1):6.