PUBLICATION
Glutathione transferase pi class 2 (GSTp2) protects against the cardiac deformities caused by exposure to PAHs but not PCB-126 in zebrafish embryos
- Authors
- Garner, L.V., and Di Giulio, R.T.
- ID
- ZDB-PUB-120125-43
- Date
- 2012
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 155(4): 573-579 (Journal)
- Registered Authors
- Di Giulio, Richard T.
- Keywords
- glutathione transferase, morpholino, polycyclic aromatic hydrocarbons, polychlorinated biphenyls, zebrafish, cardiotoxicity
- MeSH Terms
-
- Drug Therapy, Combination
- Zebrafish
- Gene Expression Regulation, Developmental/drug effects
- Animals
- Heart Defects, Congenital*/chemically induced
- Heart Defects, Congenital*/metabolism
- Heart Defects, Congenital*/prevention & control
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/enzymology
- Drug Synergism
- Polychlorinated Biphenyls/toxicity*
- Estrogen Antagonists/toxicity*
- Polycyclic Aromatic Hydrocarbons/classification
- Polycyclic Aromatic Hydrocarbons/toxicity*
- Embryonic Development/drug effects*
- Morpholinos/administration & dosage
- Morpholinos/genetics
- Gene Silencing/drug effects
- Gene Silencing/physiology
- Glutathione S-Transferase pi/genetics
- Glutathione S-Transferase pi/metabolism*
- Gene Knockdown Techniques
- PubMed
- 22269188 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
- CTD
- 22269188
Citation
Garner, L.V., and Di Giulio, R.T. (2012) Glutathione transferase pi class 2 (GSTp2) protects against the cardiac deformities caused by exposure to PAHs but not PCB-126 in zebrafish embryos. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 155(4):573-579.
Abstract
Glutathione transferases (GSTs) are phase II enzymes that detoxify a wide range of toxicants and reactive intermediates. One such class of toxicants is the ubiquitous polycyclic aromatic hydrocarbons (PAHs). Certain PAHs are known to cause developmental cardiac toxicity in fish. Herein, we explored the role of GST pi class 2 (GSTp2) in PAH- and PCB-induced cardiac toxicity in zebrafish (Danio rerio) embryos. We measured expression of GSTp2 in embryos exposed to individual and co-exposures of the PAHs benzo[k]fluoranthene (BkF), benzo[a]pyrene (BaP), and fluoranthene (FL) as well as 3,32,4,42,5-pentachlorobiphenyl (PCB-126). GSTp2 mRNA expression was induced by exposure to BkF, BaP, PCB-126, and BaP + FL and BkF + FL co-exposure. A splice junction morpholino was then used to knockdown GSTp2 in developing zebrafish. GSTp2 knockdown exacerbated the toxicity caused by co-exposures to BkF + FL and BaP + FL. However, GSTp2 knockdown did not affect PCB-126 toxicity. These results further suggest that pi class GSTs serve a protective function against the synergistic toxicity caused by PAHs in developing zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping