ZFIN ID: ZDB-PUB-120125-30
Imaging the Sialome during Zebrafish Development with Copper-Free Click Chemistry
Dehnert, K.W., Baskin, J.M., Laughlin, S.T., Beahm, B.J., Naidu, N.N., Amacher, S.L., and Bertozzi, C.R.
Date: 2012
Source: Chembiochem : a European journal of chemical biology   13(3): 353-357 (Journal)
Registered Authors: Amacher, Sharon
Keywords: bioorthogonal chemistry, embryonic development, fluorescence imaging, glycosylation, sialic acids
MeSH Terms:
  • Animals
  • Click Chemistry*
  • Copper
  • Glycolipids/analysis*
  • Glycolipids/chemistry
  • Glycolipids/metabolism
  • Sialic Acids/analysis
  • Sialic Acids/chemistry
  • Sialic Acids/metabolism*
  • Staining and Labeling*
  • Zebrafish/embryology*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/analysis*
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/metabolism
PubMed: 22262667 Full text @ Chembiochem

The sialome is comprised of sialylated glycoproteins and glycolipids that play essential roles in cell–cell communication. Using azide-modified molecular precursors of sialic acids and copper-free click chemistry, we visualized the spatiotemporal dynamics of the sialome in live zebrafish embryos.