PUBLICATION
Imaging the Sialome during Zebrafish Development with Copper-Free Click Chemistry
- Authors
- Dehnert, K.W., Baskin, J.M., Laughlin, S.T., Beahm, B.J., Naidu, N.N., Amacher, S.L., and Bertozzi, C.R.
- ID
- ZDB-PUB-120125-30
- Date
- 2012
- Source
- Chembiochem : a European journal of chemical biology 13(3): 353-357 (Journal)
- Registered Authors
- Amacher, Sharon
- Keywords
- bioorthogonal chemistry, embryonic development, fluorescence imaging, glycosylation, sialic acids
- MeSH Terms
-
- Animals
- Click Chemistry*
- Copper
- Glycolipids/analysis*
- Glycolipids/chemistry
- Glycolipids/metabolism
- Sialic Acids/analysis
- Sialic Acids/chemistry
- Sialic Acids/metabolism*
- Staining and Labeling*
- Zebrafish/embryology*
- Zebrafish/metabolism*
- Zebrafish Proteins/analysis*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/metabolism
- PubMed
- 22262667 Full text @ Chembiochem
Citation
Dehnert, K.W., Baskin, J.M., Laughlin, S.T., Beahm, B.J., Naidu, N.N., Amacher, S.L., and Bertozzi, C.R. (2012) Imaging the Sialome during Zebrafish Development with Copper-Free Click Chemistry. Chembiochem : a European journal of chemical biology. 13(3):353-357.
Abstract
The sialome is comprised of sialylated glycoproteins and glycolipids that play essential roles in cell–cell communication. Using azide-modified molecular precursors of sialic acids and copper-free click chemistry, we visualized the spatiotemporal dynamics of the sialome in live zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping