PUBLICATION

Regulation of spinal interneuron differentiation by the paracrine action of glycine

Authors
Côté, S., and Drapeau, P.
ID
ZDB-PUB-120112-3
Date
2012
Source
Developmental Neurobiology   72(2): 208-214 (Journal)
Registered Authors
Drapeau, Pierre
Keywords
glycine receptors, KCC2, interneurons, motor neurons, sensory neurons
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Basic Helix-Loop-Helix Transcription Factors
  • Cell Differentiation/drug effects
  • Cell Differentiation/physiology*
  • ELAV Proteins/metabolism
  • Glutamate Decarboxylase/metabolism
  • Glycine/metabolism*
  • Glycine Agents/pharmacology
  • Green Fluorescent Proteins
  • Homeodomain Proteins/metabolism
  • Interneurons/physiology*
  • Microscopy, Confocal
  • Morpholinos/pharmacology
  • Nerve Tissue Proteins
  • PAX2 Transcription Factor/metabolism
  • Paracrine Communication/physiology*
  • Receptors, Glycine/deficiency
  • Signal Transduction/drug effects
  • Signal Transduction/physiology*
  • Spinal Cord/cytology*
  • Strychnine/pharmacology
  • Symporters
  • Xenopus Proteins
  • Zebrafish/embryology
  • Zebrafish Proteins/metabolism
PubMed
22234938 Full text @ Dev. Neurobiol.
Abstract

Glycine and γ-aminobutyric acid (GABA) are depolarizing during early development but the purpose is unclear. We tested the effect of altering glycine signaling in zebrafish embryos by overexpressing the potassium-chloride co-transporter type 2 (KCC2) to reverse the chloride gradient or by blocking glycine receptors with strychnine or by selectively knocking down the embryonic glycine receptor (GlyR KD). Using a variety of markers we observed in all three cases a reduction of all types of spinal interneuron populations examined, indicating that glycine modulates their overall differentiation rather than choice of cell fate. Other cell populations (motor, sensory, and glial cells) were unaffected. As glycine appeared to act preceding neural and synaptic development, we examined the bandoneon (beo) mutant in which glycine receptors are functional but not clustered at synapses. Neural populations in beo embryos appeared normal, suggesting a paracrine action of circulating glycine in promoting interneuron differentiation.

Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes