Evolutionary history of c-myc in teleosts and characterization of the duplicated c-myca genes in goldfish embryos
- Authors
- Marandel, L., Labbe, C., Bobe, J., and Le Bail, P.Y.
- ID
- ZDB-PUB-120111-9
- Date
- 2012
- Source
- Molecular reproduction and development 72(2): 85-96 (Journal)
- Registered Authors
- Bobe, Julien
- Keywords
- none
- MeSH Terms
-
- Goldfish/embryology
- Goldfish/genetics*
- Synteny
- Gene Duplication*/physiology
- Evolution, Molecular*
- Embryo, Nonmammalian
- Protein Isoforms/genetics
- Protein Isoforms/metabolism
- Sequence Homology, Nucleic Acid
- Zebrafish/genetics
- Zebrafish/metabolism
- Molecular Sequence Data
- Gene Expression Regulation, Developmental
- Genome
- Animals
- Fishes/embryology
- Fishes/genetics*
- Sequence Analysis, DNA
- Phylogeny
- Genes, myc/genetics*
- PubMed
- 22213278 Full text @ Mol. Reprod. Dev.
c-Myc plays an important role during embryogenesis in mammals, but little is known about its function during embryonic development in teleosts. In addition, the evolutionary history of c-myc gene in teleosts remains unclear, and depending on the species, a variable number of gene duplicates exist in teleosts. To gain new insight into c-myc genes in teleosts, the present study was designed to clarify the evolutionary history of c-myc gene(s) in teleosts and to subsequently characterize DNA methylation and early embryonic expression patterns in a cyprinid fish. Our results show that a duplication of c-myc gene occurred before or around the teleost radiation, as a result of the teleost-specific whole genome duplication giving rise to c-myca and c-mycb in teleosts and was followed by a loss of the c-mycb gene in the Gasterosteiforms and Tetraodontiforms. Our data also demonstrate that both c-myc genes previously identified in carp and goldfish are co-orthologs of the zebrafish c-myca. These results indicate the presence of additional c-myca duplication in Cyprininae. We were able to identify differences between the expression patterns of the two goldfish c-myca genes in oocytes and early embryos. These differences suggest a partial sub-functionalization of c-myca genes after duplication. Despite differences in transcription patterns, both of the c-myca genes displayed similar DNA methylation patterns during early development and in gametes. Together, our results clarify the evolutionary history of the c-myc gene in teleosts and provide new insight into the involvement of c-myc in early embryonic development in cyprinids.