PUBLICATION
Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish
- Authors
- Hu, S.N., Yu, H., Zhang, Y.B., Wu, Z.L., Yan, Y.C., Li, Y.X., Li, Y.Y., and Li, Y.P.
- ID
- ZDB-PUB-120111-3
- Date
- 2012
- Source
- FEBS letters 586(3): 222-228 (Journal)
- Registered Authors
- Keywords
- sox31, MBT, apoptosis
- Datasets
- GEO:GSE32914
- MeSH Terms
-
- Apoptosis/drug effects
- Apoptosis/genetics*
- Zebrafish Proteins/genetics*
- RNA Splicing/drug effects*
- Alternative Splicing/drug effects
- Mothers
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Zygote/cytology
- Zygote/drug effects*
- Zygote/metabolism
- Zebrafish/embryology*
- Female
- Gene Expression Regulation, Developmental/drug effects
- Gene Expression Regulation, Developmental/genetics
- Blastula/cytology*
- Blastula/drug effects
- Blastula/metabolism*
- Morpholinos/pharmacology
- SOX Transcription Factors/genetics*
- Animals
- PubMed
- 22209980 Full text @ FEBS Lett.
Citation
Hu, S.N., Yu, H., Zhang, Y.B., Wu, Z.L., Yan, Y.C., Li, Y.X., Li, Y.Y., and Li, Y.P. (2012) Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish. FEBS letters. 586(3):222-228.
Abstract
Here we report that splice blocking morpholinos (Sb MO) against zebrafish sox31 elicit developmental arrest, likely through creating a series of dominant negative splicing variants. Embryos injected with the Sb MO develop normally before the Mid-Blastula Transition (MBT); however, they do not initiate epiboly. Microarray analysis of mRNAs collected at the dome stage revealed that the Sb MO impairs activation of a large set of zygotic genes and reduces degradation of maternal mRNA during MBT. Furthermore, an apoptotic response occurs in Sb morphants at about 6 hpf. SoxB1 family genes including sox31 thus play an essential role for early embryos traversing the transitional stage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping