PUBLICATION

Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish

Authors
Hu, S.N., Yu, H., Zhang, Y.B., Wu, Z.L., Yan, Y.C., Li, Y.X., Li, Y.Y., and Li, Y.P.
ID
ZDB-PUB-120111-3
Date
2012
Source
FEBS letters   586(3): 222-228 (Journal)
Registered Authors
Keywords
sox31, MBT, apoptosis
Datasets
GEO:GSE32914
MeSH Terms
  • Morpholinos/pharmacology
  • Mothers
  • Apoptosis/drug effects
  • Apoptosis/genetics*
  • RNA Splicing/drug effects*
  • Zebrafish/embryology*
  • Zygote/cytology
  • Zygote/drug effects*
  • Zygote/metabolism
  • Blastula/cytology*
  • Blastula/drug effects
  • Blastula/metabolism*
  • Animals
  • Alternative Splicing/drug effects
  • Gene Expression Regulation, Developmental/drug effects
  • Gene Expression Regulation, Developmental/genetics
  • Female
  • Zebrafish Proteins/genetics*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • SOX Transcription Factors/genetics*
(all 21)
PubMed
22209980 Full text @ FEBS Lett.
Abstract
Here we report that splice blocking morpholinos (Sb MO) against zebrafish sox31 elicit developmental arrest, likely through creating a series of dominant negative splicing variants. Embryos injected with the Sb MO develop normally before the Mid-Blastula Transition (MBT); however, they do not initiate epiboly. Microarray analysis of mRNAs collected at the dome stage revealed that the Sb MO impairs activation of a large set of zygotic genes and reduces degradation of maternal mRNA during MBT. Furthermore, an apoptotic response occurs in Sb morphants at about 6 hpf. SoxB1 family genes including sox31 thus play an essential role for early embryos traversing the transitional stage.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
No data available
Mapping
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Citation
Hu, S.N., Yu, H., Zhang, Y.B., Wu, Z.L., Yan, Y.C., Li, Y.X., Li, Y.Y., and Li, Y.P. (2012) Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish. FEBS letters. 586(3):222-228.