PUBLICATION
Molecular cloning and partial functional characterization of a proliferation inducing ligand (APRIL) in zebrafish (Danio rerio)
- Authors
- Min, C., Liang, Z., Cui, X., Wang, Q., Chen, Y., and Zhang, S.
- ID
- ZDB-PUB-120109-2
- Date
- 2012
- Source
- Developmental and comparative immunology 37(1): 202-206 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Proliferation
- Cell Survival
- Cells, Cultured
- Cloning, Molecular
- Lymphocytes/physiology
- Molecular Sequence Data
- Phylogeny
- Recombinant Fusion Proteins/genetics*
- Recombinant Fusion Proteins/metabolism
- Recombinant Fusion Proteins/physiology
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Spleen/cytology
- Tumor Necrosis Factor Ligand Superfamily Member 13/genetics*
- Tumor Necrosis Factor Ligand Superfamily Member 13/metabolism
- Tumor Necrosis Factor Ligand Superfamily Member 13/physiology
- Zebrafish
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology
- PubMed
- 22197036 Full text @ Dev. Comp. Immunol.
Citation
Min, C., Liang, Z., Cui, X., Wang, Q., Chen, Y., and Zhang, S. (2012) Molecular cloning and partial functional characterization of a proliferation inducing ligand (APRIL) in zebrafish (Danio rerio). Developmental and comparative immunology. 37(1):202-206.
Abstract
Here we describe the identification of a Danio rerio homologue of a proliferation-inducing ligand (APRIL) of the TNF family (designated zAPRIL). Sequence analysis showed that the open reading frame of zAPRIL consists of 600 bases encoding a protein of 199-amino acids. Recombinant soluble APRIL (zsAPRIL) was constructed consisting of fluorescence-enhanced green fluorescent protein (EGFP) and cloned into a pET28a vector. SDS-PAGE and western blotting analysis indicated a high-level expression of soluble EGFP/zsAPRIL protein in Escherichia coli BL21 (DE3). Observation by confocal microscopy demonstrated that EGFP/zsAPRIL could successfully bind to the surface receptors of zebrafish lymphocytes. In vitro survival analysis revealed that purified EGFP/zsAPRIL was able to promote the survival of zebrafish lymphocytes in a dose-dependent manner. The biological role of APRIL does not seem to be restricted to proliferation induction. Zebrafish may could served as a model organism for further study of APRIL.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping