The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR
- Authors
- Zhao, S., Xie, P., Li, G., Jun, C., Cai, Y., Xiong, Q., and Zhao, Y.
- ID
- ZDB-PUB-111206-9
- Date
- 2012
- Source
- Proteomics 12(2): 300-312 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Glycolysis
- Injections, Intraperitoneal
- Intermediate Filament Proteins/metabolism
- Lethal Dose 50
- Male
- Microcystins/administration & dosage
- Microcystins/toxicity*
- Microscopy, Electron, Transmission
- Mitochondria/drug effects
- Mitochondria/metabolism
- Oxidative Stress
- Proteome/analysis*
- Proteome/metabolism
- Proteomics/methods
- Testis/drug effects*
- Testis/metabolism
- Testis/ultrastructure
- Tubulin/metabolism
- Zebrafish/metabolism*
- Zebrafish Proteins/metabolism
- PubMed
- 22140076 Full text @ Proteomics
Microcystin-RR (MC-RR) is a commonly encountered cyanotoxin and receives increasing attention due to the risk of its bioaccumulation in aquatic animals like sh. This study investigated the protein proles of zebrafish (Danio rerio) testes after intraperitoneal injection (i.p.) with 0.5 LD(50) (2000 μg/kg). MC-RR caused a noticeable damage to testicular ultrastructure, showing widened intercellular junction, distention of mitochondria. The testes showed a rapid response of its defense systems to the oxidative stress caused by MC-RR. This is the first to use proteomic approach to obtain an overview of the effects of MC- RR on the testes of zebrafish. The proteomic results revealed that toxin exposure remarkably altered abundance of 24 proteins that were took part in cytoskeleton assembly, oxidative stress, glycolysis metabolism, calcium ion binding and other biological functions. In conclusion, MC-RR damaged the testes and was toxic to the reproductive system of male zebrafish mainly through causing oxidative stress.