PUBLICATION
Real-time optical gating for three-dimensional beating heart imaging
- Authors
- Taylor, J.M., Saunter, C.D., Love, G.D., Girkin, J.M., Henderson, D.J., and Chaudhry, B.
- ID
- ZDB-PUB-111129-30
- Date
- 2011
- Source
- Journal of Biomedical Optics 16(11): 116021 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Algorithms
- Animals
- Embryo, Nonmammalian
- Heart/anatomy & histology
- Heart/embryology*
- Heart/physiology*
- Image Processing, Computer-Assisted/methods*
- Kymography
- Microscopy, Fluorescence/methods
- Myocardial Contraction/physiology
- Zebrafish
- PubMed
- 22112126 Full text @ J. Biomed. Opt.
Citation
Taylor, J.M., Saunter, C.D., Love, G.D., Girkin, J.M., Henderson, D.J., and Chaudhry, B. (2011) Real-time optical gating for three-dimensional beating heart imaging. Journal of Biomedical Optics. 16(11):116021.
Abstract
We demonstrate real-time microscope image gating to an arbitrary position in the cycle of the beating heart of a zebrafish embryo. We show how this can be used for high-precision prospective gating of fluorescence image slices of the moving heart. We also present initial results demonstrating the application of this technique to 3-D structural imaging of the beating embryonic heart.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping