PUBLICATION
The yolk cell of the zebrafish blastula harbors functional apoptosis machinery
- Authors
- Popgeorgiev, N., Prudent, J., Bonneau, B., and Gillet, G.
- ID
- ZDB-PUB-111117-6
- Date
- 2011
- Source
- Communicative & integrative biology 4(5): 549-551 (Journal)
- Registered Authors
- Gillet, Germain
- Keywords
- apoptosis, zebrafish, capases, cytochrome c release, YSL, Nrz, zBax
- MeSH Terms
- none
- PubMed
- 22046458 Full text @ Commun. Integr. Biol.
Citation
Popgeorgiev, N., Prudent, J., Bonneau, B., and Gillet, G. (2011) The yolk cell of the zebrafish blastula harbors functional apoptosis machinery. Communicative & integrative biology. 4(5):549-551.
Abstract
We recently described the implication of the Bcl-2 related antiapoptotic Nrz protein during early zebrafish development. Nrz knock-down induces calcium-dependent cytoskeleton remodeling leading to margin constriction and premature embryo lethality. In the YSL, nrz knock-down embryos exhibit some typical features of apoptosis such as mitochondrial transmembrane potential loss and cytochrome c release. However, downstream caspase-3 activation has not been detected so far. Here, we report that the YSL contains fully functional apoptotic machinery that can activate caspase-3 following zBax ectopic expression. Furthermore, we present evidence that caspase-3 activation is actually detectable in nrz knock-down embryos when premature margin constriction is prevented.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping