PUBLICATION
Hedgehog and retinoic acid signaling cooperate to promote motoneurogenesis in zebrafish
- Authors
- Mich, J.K., and Chen, J.K.
- ID
- ZDB-PUB-111117-41
- Date
- 2011
- Source
- Development (Cambridge, England) 138(23): 5113-5119 (Journal)
- Registered Authors
- Chen, James K., Mich, John
- Keywords
- hedgehog, motoneuron, retinoic acid, zebrafish
- MeSH Terms
-
- Trans-Activators/metabolism
- Tretinoin/metabolism*
- Real-Time Polymerase Chain Reaction
- Zebrafish/physiology*
- In Situ Hybridization
- Motor Neurons/physiology*
- Neurogenesis/genetics
- Neurogenesis/physiology*
- Signal Transduction/physiology*
- Receptors, G-Protein-Coupled/deficiency*
- Oligonucleotides/genetics
- Zebrafish Proteins/deficiency*
- Oncogene Proteins/metabolism
- Hedgehog Proteins/metabolism*
- DNA Primers/genetics
- Animals
- PubMed
- 22069185 Full text @ Development
Citation
Mich, J.K., and Chen, J.K. (2011) Hedgehog and retinoic acid signaling cooperate to promote motoneurogenesis in zebrafish. Development (Cambridge, England). 138(23):5113-5119.
Abstract
The precise requirements of Hedgehog (Hh) pathway activity in vertebrate central nervous system development remain unclear,
particularly in organisms with both maternally and zygotically derived signaling. Here we describe the motoneural phenotype
of zebrafish that lack maternal and zygotic contributions of the Hh signaling transducer Smoothened (MZsmo mutants) and therefore are completely devoid of ligand-dependent pathway activation. Some functional primary motoneurons
(PMNs) persist in the absence of Hh signaling, and we find that their induction requires both basal Gli transcription factor
activity and retinoic acid (RA) signaling. We also provide evidence that RA pathway activation can modulate Gli function in
a Hh ligand-independent manner. These findings support a model in which Hh and RA signaling cooperate to promote PMN cell
fates in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping