ZFIN ID: ZDB-PUB-111117-25
Coagulation factor III (tissue factor) is required for vascularization in zebrafish embryos
Zhou, R.F., Liu, Y., Wang, Y.X., Mo, W., and Yu, M.
Date: 2011
Source: Genetics and molecular research : GMR   10(4): 4147-4157 (Journal)
Registered Authors:
Keywords: tissue factor, vasculature, brain development, zebrafish
MeSH Terms:
  • Animals
  • Blood Coagulation/physiology
  • Embryo, Nonmammalian/blood supply*
  • Embryo, Nonmammalian/metabolism
  • Factor XIII/genetics
  • Factor XIII/metabolism*
  • Gene Knockout Techniques
  • Neovascularization, Pathologic/metabolism
  • Phenotype
  • Thromboplastin/genetics
  • Thromboplastin/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 22057990 Full text @ Genet. Mol. Res.
Tissue factor (coagulation factor III) is a cell surface receptor for coagulation factor VII/VIIa; it was initially recognized as an initiator of the extrinsic coagulation pathway. Recently, the zebrafish tissue factor gene (TF) has been cloned. Paralogs encode coagulation factors IIIa and IIIb; both show remarkable sequence identity to the human and mouse coagulation factor III gene. It has been reported that TF could have additional properties that are essential for normal embryonic development, since knockout of the murine coagulation factor III gene resulted in 90% embryonic lethality. We examined the role of coagulation factor IIIb (f3b) during zebrafish embryonic development. Expression analysis revealed that endogenous f3b was chronologically expressed in the pectoral fins and in the vicinity of the pharynx. Knockout of f3b by injection of an f3b morpholino at the one-to-two cell stage caused distinctive morphological defects in embryos, including edema in the fourth brain ventricle at early embryonic stages and occasional bleeding at later stages. Furthermore, f3b morphants displayed abnormal vascular patterning. We conclude that f3b is required for brain vascular development and for development of part of the somatic vasculature during embryogenesis in the zebrafish.