Molecular cloning and functional analysis of scavenger receptor zebrafish CL-P1
- Authors
- Fukuda, M., Ohtani, K., Jang, S.J., Yoshizaki, T., Mori, K.I., Motomura, W., Yoshida, I., Suzuki, Y., Kohgo, Y., and Wakamiya, N.
- ID
- ZDB-PUB-111027-18
- Date
- 2011
- Source
- Biochimica et biophysica acta. General subjects 1810(12): 1150-9 (Journal)
- Registered Authors
- Keywords
- Collectin, Scavenger receptor, innate immunity, zebrafish, embryogenesis
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- CHO Cells
- Cloning, Molecular
- Cricetinae
- Cricetulus
- DNA, Complementary
- Gene Knockdown Techniques
- Immunohistochemistry
- In Situ Hybridization
- Molecular Sequence Data
- Polymerase Chain Reaction
- Receptors, Scavenger/genetics*
- Receptors, Scavenger/physiology
- Zebrafish
- PubMed
- 22001438 Full text @ BBA General Subjects
Background
Scavenger receptors are generally expressed in macrophages and vascular endothelial cells and some scavenger receptors are thought to contribute to the development of atherosclerosis.
Methods
We cloned the cDNA of a zebrafish CL-P1 (collectin placenta 1) and performed a knockdown study using its antisense morpholino oligonucleotides (MO).
Results
Zebrafish CL-P1 (zCL-P1) is 51% identical to human CL-P1 in its amino acid sequence. Microbes and OxLDL bound to zCL-P1 cDNA transfected cells. zCL-P1 mRNA expression gradually increased after 6 hours post-fertilization (hpf), reached its highest level at 24 hpf, and then decreased, which is similar to the gene expression pattern of Tie-2. The knockdown of zCL-P1 led to an increase in the number of zebrafish embryos with severe morphological abnormalities such as short body lengths and defects in the dorsal aorta at 48 hpf. Simultaneous injection of both MO and synthetic zCL-P1 or zVEGF mRNA rescued the abnormal phenotype.
Conclusions
In vivo knockdown study shows that zCL-P1 is implicated in vasculogenesis and those of our in vitro study support its role as a scavenger receptor.
General significance
These results suggest that zCL-P1 might be essential for vasculogenesis during the early embryonic phase in bone fish.