PUBLICATION
Fluorescent imaging of cancer in zebrafish
- Authors
- Ignatius, M.S., and Langenau, D.M.
- ID
- ZDB-PUB-111012-23
- Date
- 2011
- Source
- Methods in cell biology 105: 437-459 (Chapter)
- Registered Authors
- Ignatius, Myron, Langenau, David
- Keywords
- confocal microscopy, irradiated, labeling, mutagenesis, recipient, regulation
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Tracking/methods
- Cell Transformation, Neoplastic/genetics
- Cell Transformation, Neoplastic/metabolism
- Cell Transformation, Neoplastic/ultrastructure
- Fluorescent Dyes/analysis
- Green Fluorescent Proteins/genetics*
- Green Fluorescent Proteins/metabolism
- Image Processing, Computer-Assisted/methods
- Medical Oncology/methods*
- Microscopy, Confocal
- Microscopy, Fluorescence/methods*
- Molecular Imaging/methods*
- Mosaicism
- Mutagenesis
- Mutation
- Neoplasm Transplantation/methods*
- Neoplasms/genetics*
- Neoplasms/metabolism
- Neoplasms/pathology
- Spectrometry, Fluorescence
- Whole-Body Irradiation
- Zebrafish/anatomy & histology*
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 21951542 Full text @ Meth. Cell. Biol.
Citation
Ignatius, M.S., and Langenau, D.M. (2011) Fluorescent imaging of cancer in zebrafish. Methods in cell biology. 105:437-459.
Abstract
Zebrafish are an ideal model organism to research cancer. Zebrafish embryos and larvae are optically translucent, which has made imaging multiple processes in development and disease possible. When coupled with fluorescent imaging techniques, zebrafish are fast becoming a model of choice for following tumor formation. This is highlighted by recent studies using fluorescent proteins to image xenograft transplantation, neovascularization, growth responses to drug treatments, and self-renewal. Fluorescent labeled tumors can be generated in zebrafish by multiple methods including chemical mutagenesis, oncogene expression by mosaic or stable transgenesis, or genetic mutations that are predisposing to cancer. In this chapter, we highlight the studies that have employed fluorescence to follow critical aspects of tumorigenesis, with particular focus on providing methods for labeling, isolating, transplanting, and imaging fluorescently labeled tumors in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping