PUBLICATION

Function of strawberry notch Family Genes in the Zebrafish Brain Development

Authors
Takano, A., Zochi, R., Hibi, M., Terashima, T., and Katsuyama, Y.
ID
ZDB-PUB-111011-8
Date
2011
Source
The Kobe journal of medical sciences   56(5): E220-E230 (Journal)
Registered Authors
Hibi, Masahiko
Keywords
zebrafish, brain development, strawberry notch, gene expression
MeSH Terms
  • Animals
  • Brain/embryology*
  • Brain/metabolism
  • ELAV Proteins/genetics
  • ELAV Proteins/metabolism
  • ELAV-Like Protein 3
  • Gene Expression
  • Gene Knockdown Techniques
  • Intracellular Signaling Peptides and Proteins/genetics
  • Intracellular Signaling Peptides and Proteins/metabolism
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Morphogenesis*
  • Repressor Proteins/genetics*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed
21937870
Abstract
We previously reported embryonic expression pattern of strawberry notch (sbno) family genes, suggesting involvement in brain development. However function of sbno genes in the vertebrate development has not been known yet. Utilizing zebrafish embryos, we experimentally examined function of sbno genes during brain development in this report. Knockdown experiments of sbno1 and sbno2a disrupted brain morphology, and delayed developmental alteration of gene expression. The earliest effect of loss of function of sbno genes on the zebrafish embryogenesis that we found here was downregulation of otx2 expression. Knockdown of sbno1 specifically affects regionalization along the anterior-posterior axis of the brain. These results suggest essential roles of sbno genes in vertebrate brain development.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping