Expression characterization and the promoter activity analysis of zebrafish hdac4
- Authors
- Zhu, K., Wang, H., Gul, Y., Zhao, Y., Wang, W., Liu, S., and Wang, M.
- ID
- ZDB-PUB-110927-2
- Date
- 2012
- Source
- Fish physiology and biochemistry 38(2): 585-593 (Journal)
- Registered Authors
- Keywords
- hdac4, zebrafish, TSA, expression, promoter activity
- MeSH Terms
-
- Embryonic Development/drug effects
- Hydroxamic Acids/pharmacology
- Zebrafish Proteins/metabolism*
- Base Sequence
- Fish Proteins/antagonists & inhibitors
- Fish Proteins/metabolism*
- Histone Deacetylase Inhibitors/pharmacology
- Promoter Regions, Genetic*
- NIH 3T3 Cells
- Myogenic Regulatory Factors/antagonists & inhibitors
- Myogenic Regulatory Factors/metabolism*
- MEF2 Transcription Factors
- Zebrafish/genetics
- Zebrafish/metabolism*
- Molecular Sequence Data
- Cricetinae
- Mice
- Animals
- Histone Deacetylases/metabolism*
- PubMed
- 21773810 Full text @ Fish Physiol. Biochem.
Histone deacetylase 4 (HDAC4) is an important modifier enzyme for chromatin remodeling and plays an essential role in regulating gene expression. Spatio-temporal expression spectrum revealed that zebrafish hdac4 mRNA, ubiquitously distributed in various tissues, were significantly higher at 36 hpf (hours post-fertilization) and 6 dpf (days post-fertilization) than other periods. Trichostatin A (TSA) inhibited the development of zebrafish embryos and transcription of hdac4 and mef2a (myocyte enhancer factor-2A). Moreover, five vectors containing different promoter regions of hdac4 were constructed in order to analyze promoter activity. The vector containing the region from -125 to +160 exhibited maximum luciferase activity that was approximately 30.3-fold and 58.9-fold higher than the control in two kinds of cells, respectively. By comparing the luciferase activities between the region from -302 to +30 and -698 to +30, it was suggested that the region between -698 and -302 might contain mild negative regulatory elements.