PUBLICATION
Genetic suppressor screens in haploids
- Authors
- Bai, X., Yang, Z., Jiang, H., Lin, S., and Zon, L.I.
- ID
- ZDB-PUB-110921-9
- Date
- 2011
- Source
- Methods in cell biology 104: 129-136 (Chapter)
- Registered Authors
- Bai, Xiaoying, Lin, Shuo, Yang, Zhongan, Zon, Leonard I.
- Keywords
- suppressor screen, modifier screen, haploid, BAC transgene, ENU mutagenesis
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Chromosomes, Artificial, Bacterial/genetics
- DNA Mutational Analysis
- Ethylnitrosourea/pharmacology
- Female
- Genes, Recessive
- Genes, Suppressor*
- Genetic Testing/methods*
- Haploidy
- Larva/genetics
- Male
- Mutagenesis
- Mutagens/pharmacology
- Mutation
- Transcription Factors/genetics
- Zebrafish/genetics*
- Zebrafish Proteins/genetics
- PubMed
- 21924160 Full text @ Meth. Cell. Biol.
Citation
Bai, X., Yang, Z., Jiang, H., Lin, S., and Zon, L.I. (2011) Genetic suppressor screens in haploids. Methods in cell biology. 104:129-136.
Abstract
As a vertebrate genetic model, the zebrafish has been well recognized for its strength in studying a variety of biological processes and human diseases. Traditional forward genetic screens in zebrafish have generated a large pool of mutants with interesting phenotypes resembling human diseases but the underlying mechanisms are not well understood. A powerful approach to elucidate the mechanisms of these mutants is the modifier screen, which identifies 2(nd)-site mutations that specifically enhance or block the phenotype of a given mutant. Here we described the first genetic suppressor screen in zebrafish, which identifies a novel transcriptional mechanism regulating erythropoiesis. In combination with the haploid genetics in zebrafish, we have shown the feasibility and strength of a modifier screen in zebrafish. This strategy will greatly broaden the utility of the zebrafish as a model for making original discoveries and establishing novel paradigms for understanding vertebrate biology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping