PUBLICATION
Insights into the antibacterial and immunomodulatory functions of the antimicrobial peptide, epinecidin-1, against Vibrio vulnificus infection in zebrafish
- Authors
- Pan, C.Y., Wu, J.L., Hui, C.F., Lin, C.H., and Chen, J.Y.
- ID
- ZDB-PUB-110921-29
- Date
- 2011
- Source
- Fish & shellfish immunology 31(6): 1019-25 (Journal)
- Registered Authors
- Chen, Jyh-Yih, Wu, Jen-Leih
- Keywords
- epinecidin-1, vibrio vulnificus
- MeSH Terms
-
- Animals
- Antimicrobial Cationic Peptides/immunology
- Antimicrobial Cationic Peptides/pharmacology*
- Antimicrobial Cationic Peptides/therapeutic use
- DNA Primers/genetics
- Fish Proteins/immunology
- Fish Proteins/pharmacology*
- Fish Proteins/therapeutic use
- Gene Expression Regulation/drug effects
- Gene Expression Regulation/immunology*
- Immunomodulation/immunology*
- Interferon-gamma/metabolism
- Interleukin-10/metabolism
- Interleukin-1beta/metabolism
- Microarray Analysis
- Real-Time Polymerase Chain Reaction
- Survival Analysis
- Tumor Necrosis Factor-alpha/metabolism
- Vibrio Infections/immunology*
- Vibrio Infections/prevention & control*
- Zebrafish
- PubMed
- 21925271 Full text @ Fish Shellfish Immunol.
Citation
Pan, C.Y., Wu, J.L., Hui, C.F., Lin, C.H., and Chen, J.Y. (2011) Insights into the antibacterial and immunomodulatory functions of the antimicrobial peptide, epinecidin-1, against Vibrio vulnificus infection in zebrafish. Fish & shellfish immunology. 31(6):1019-25.
Abstract
In the present study, we used Vibrio vulnificus and a zebrafish model system to investigate the inhibitory effect of epinecidin-1 on acute bacterial infection and studied the impacts of pretreatment, co-treatment, and post-treatment with epinecidin-1 on its protective efficacy. In vivo experiments showed that co-treatment with epinecidin-1 and V. vulnificus achieved 78%–97% survival rates after 30 days. When epinecidin-1 and V. vulnificus were co-injected into zebrafish and zebrafish were re-challenged with V. vulnificus after 30 days, zebrafish had survival rates of 22%–47%. Pretreatment and post-treatment with epinecidin-1 obtained respective survival rates of 57% and 60%. In addition, epinecidin-1 modulated the expressions of immune-responsive genes like interleukin (IL)-10, IL-1b, tumor necrosis factor-α, and interferon-γ as analyzed by a microarray and qPCR approach. This study demonstrates the use of epinecidin-1 to develop inactivated material for fish bacterial infections which can provide guidelines for the future design of epinecidin-1-bacterial formulations for various in vivo applications.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping