PUBLICATION
The Zon laboratory guide to positional cloning in zebrafish (2011)
- Authors
- Zhou, Y., and Zon, L.I.
- ID
- ZDB-PUB-110921-18
- Date
- 2011
- Source
- Methods in cell biology 104: 287-309 (Chapter)
- Registered Authors
- Zhou, Yi, Zon, Leonard I.
- Keywords
- positional cloning, mutant mapping, polymorphism, SNP, allele, microsatellite marker
- MeSH Terms
-
- Animal Husbandry/methods
- Animals
- Chromosome Mapping/methods*
- Cloning, Molecular/methods*
- DNA/isolation & purification
- Female
- Genetic Association Studies/methods
- Genetic Linkage
- Genetic Markers
- Humans
- Larva/genetics
- Male
- Mutation
- Phenotype
- Polymorphism, Single Nucleotide
- Real-Time Polymerase Chain Reaction/methods
- Sequence Analysis, DNA
- Synteny
- Takifugu/genetics
- Zebrafish/genetics*
- PubMed
- 21924169 Full text @ Meth. Cell. Biol.
Citation
Zhou, Y., and Zon, L.I. (2011) The Zon laboratory guide to positional cloning in zebrafish (2011). Methods in cell biology. 104:287-309.
Abstract
Zebrafish genome sequencing project has improved efficiency of positional cloning in zebrafish and traditional chromosome walking by isolating large insert genomic libraries has become a past. However, the genetic principles underlying the positional cloning still form the foundation for current chromosome walking using the genome sequence assemblies and related genomic sequence and clone information. This guide intends to summarize our accumulated experience in positional cloning using the current genomic resources and tools, and provide a practical guide to positional and/or candidate cloning of mutants of interest.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping