PUBLICATION
The Immunohistochemical Patterns of Calcification-related Molecules in the Epidermis and Dermis of the Zebrafish (Danio rerio)
- Authors
- Hong, Y.J., Choi, Y.W., Myung, K.B., and Choi, H.Y.
- ID
- ZDB-PUB-110920-8
- Date
- 2011
- Source
- Annals of dermatology 23(3): 299-303 (Journal)
- Registered Authors
- Keywords
- calcification, elasmoid scale, zebrafish
- MeSH Terms
- none
- PubMed
- 21909199 Full text @ Ann. Dermatol.
Citation
Hong, Y.J., Choi, Y.W., Myung, K.B., and Choi, H.Y. (2011) The Immunohistochemical Patterns of Calcification-related Molecules in the Epidermis and Dermis of the Zebrafish (Danio rerio). Annals of dermatology. 23(3):299-303.
Abstract
Background
The scales of bony fish represent a significant reservoir of calcium and calcification of the elasmoid scale is known to be associated with deposition of mineral crystals from the epidermis to dermis. However, little is known about the exact mechanisms of calcium deposition, mobilization and regeneration occurring in the zebrafish skin.
Objective
The purpose of this study was to investigate the expression of calcification-related molecular mediators in both the epidermis and dermis of the zebrafish (Danio rerio), using immunohistochemical study.
Methods
We examined the skin of zebrafish in four populations of different ages (i.e. 20 days post-fertilization (dpf), 35 dpf, 50 dpf, and the adult zebrafish), using several immunohistochemical markers, including bone morphogenetic protein 4 (BMP-4), β-catenin, osteocalcin, osteopontin and osteonectin.
Results
BMP-4, osteopontin and osteonectin were moderately expressed in the epidermis of zebrafish after 35 dpf. Also, some of the cells in the upper dermis showed strong positivity for BMP-4, osteocalcin, osteopontin and osteonetin.
Conclusion
Our results suggest that BMP-4, osteocalcin, osteopontin and osteonectin may play a role in the process of calcification of the elasmoid scale.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping