PUBLICATION
PTZ-induced seizures inhibit adenosine deamination in adult zebrafish brain membranes
- Authors
- Siebel, A.M., Piato, A.L., Capiotti, K.M., Seibt, K.J., Bogo, M.R., and Bonan, C.D.
- ID
- ZDB-PUB-110920-5
- Date
- 2011
- Source
- Brain research bulletin 86(5-6): 385-9 (Journal)
- Registered Authors
- Bonan, Carla Denise
- Keywords
- adenosine, adenosine deaminase, ectonucleotidases, seizures, pentylenetetrazole, zebrafish
- MeSH Terms
-
- Adenosine/metabolism*
- Adenosine Deaminase/metabolism
- Animals
- Brain/anatomy & histology*
- Brain/metabolism*
- Convulsants/pharmacology*
- Deamination
- Pentylenetetrazole/pharmacology*
- Pyrophosphatases/metabolism
- Seizures/chemically induced*
- Seizures/physiopathology*
- Zebrafish
- PubMed
- 21907764 Full text @ Brain Res. Bull.
Citation
Siebel, A.M., Piato, A.L., Capiotti, K.M., Seibt, K.J., Bogo, M.R., and Bonan, C.D. (2011) PTZ-induced seizures inhibit adenosine deamination in adult zebrafish brain membranes. Brain research bulletin. 86(5-6):385-9.
Abstract
Adenosine exerts neuromodulatory functions with mostly inhibitory effects, being considered an endogenous anticonvulsant. The hydrolysis of ATP by ectonucleotidases is an important source of adenosine, and adenosine deaminase (ADA) contributes to the regulation of this nucleoside concentration through its deamination. In this study, we tested the effect of pentylenetetrazole (PTZ)-induced seizures on ectonucleotidases and ADA activities in adult zebrafish brain. Our results have demonstrated that PTZ treatments did not alter ectonucleotidases and ADA activities in membranes and soluble fraction, respectively. However, ecto-ADA activity was significantly decreased in brain membranes of animals exposed to 5 mM and 15 mM PTZ treatments (22.4% and 29.5%, respectively) when compared to the control group. Semiquantitative RT-PCR analysis did not show significant changes after the PTZ exposure on ADA gene expression. The decreased adenosine deamination observed in this study suggests a modulation of extracellular adenosine levels during PTZ-induced seizures in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping