ZFIN ID: ZDB-PUB-110920-1
Indentification and molecular characterization of the interleukin-10 receptor 1 of the zebrafish (Danio rerio) and the goldfish (Carassius auratus L.)
Grayfer, L., and Belosevic, M.
Date: 2012
Source: Developmental and comparative immunology   36(2): 408-17 (Journal)
Registered Authors:
Keywords: interleukin-10 receptor, IL10R1, interleukin-10 receptor, alpha chain, bony fish, goldfish, inflammation, gene expression, macrophages, STAT3, JAK1
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular/methods
  • Evolution, Molecular
  • Female
  • Goldfish/genetics
  • Goldfish/immunology*
  • Interleukin-10 Receptor alpha Subunit/genetics*
  • Interleukin-10 Receptor alpha Subunit/immunology*
  • Male
  • Molecular Sequence Data
  • Phylogeny
  • RNA/chemistry
  • RNA/genetics
  • Random Amplified Polymorphic DNA Technique
  • Recombinant Proteins/immunology
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Zebrafish/genetics
  • Zebrafish/immunology*
PubMed: 21906622 Full text @ Dev. Comp. Immunol.
This is the first report of the identification and molecular characterization of an interleukin-10 receptor 1 in bony fish. By gene synteny analysis, we identified the zebrafish interleukin-10 receptor 1 (IL10R1) and using this IL10R1 sequence, we cloned the goldfish IL10R1 cDNA transcript. The identified fish IL10R1 protein sequences had a putative JAK1 binding site, only one of the two STAT3 binding sites, that are present in all other vertebrates IL10R1 proteins as well as C-terminal serine rich areas, believed to be responsible for the anti-inflammatory properties of IL10R1. Phylogenetically, the fish IL10R1 proteins grouped independently of the amphibian, avian and mammalian IL10R1s. Quantitative gene expression analysis of the IL10R1 of zebrafish and goldfish revealed highest mRNA levels in the spleen tissues. High mRNA levels were also observed in the zebrafish muscle in contrast to low mRNA levels in the muscle of the goldfish. Moderate IL10R1 mRNA levels were seen in most other tissues examined and lowest gene expression was in the liver of both fish species. Goldfish monocytes stimulated with a recombinant goldfish interleukin-10 (rgIL-10) or with heat killed fish pathogens, Aeromonas salmonicida or Trypanosoma carassii, exhibited significantly reduced mRNA levels of the IL10R1. Furthermore, we produced a recombinant form of the goldfish IL10R1 (rgIL10R1) and using in vitro binding studies, demonstrated that the dimerized rgIL-10 specifically interacted with rgIL10R1. Our results suggest that interleukin-10 system has been highly conserved throughout evolution.